EP 2655611 A1 20131030 - METHOD FOR COVALENT IMMOBILIZATION OF ENZYMES ON FUNCTIONALIZED SOLID POLYMERIC SUPPORTS
Title (en)
METHOD FOR COVALENT IMMOBILIZATION OF ENZYMES ON FUNCTIONALIZED SOLID POLYMERIC SUPPORTS
Title (de)
VERFAHREN ZUR KOVALENTEN IMMOBILISIERUNG VON ENZYMEN AUF FUNKTIONALISIERTEN FESTEN POLYMERTRÄGERN
Title (fr)
PROCÉDÉ POUR L'IMMOBILISATION COVALENTE D'ENZYMES SUR DES SUPPORTS POLYMÈRES SOLIDES FONCTIONNALISÉS
Publication
Application
Priority
- IT PD20100392 A 20101223
- EP 2011073794 W 20111222
Abstract (en)
[origin: WO2012085206A1] It is herein described a method of immobilization of enzymes, in particular enzymes that are active at the interface with hydrophobic phases as lipases, on porous solid polymeric supports, in which there is formation of a stable covalent bond between the support and the enzyme and in which a hydrophobic "interface" forms around the solid support that allows the enzyme, dissolved in a minimum aqueous phase, to pass from the closed/inactive conformation (which prevails when the enzyme is in aqueous phase) to the open/activated conformation (which prevails when the enzyme comes into contact with a hydrophobic phase) and to adopt an optimum orientation, turning the opening of the hydrophobic active site towards the hydrophobic organic phase.
IPC 8 full level
C12N 11/02 (2006.01); C12N 11/04 (2006.01)
CPC (source: EP US)
C12N 9/20 (2013.01 - EP); C12N 11/087 (2020.01 - EP US)
Citation (search report)
See references of WO 2012085206A1
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
DOCDB simple family (publication)
WO 2012085206 A1 20120628; EP 2655611 A1 20131030; IT 1403355 B1 20131017; IT PD20100392 A1 20120624
DOCDB simple family (application)
EP 2011073794 W 20111222; EP 11811024 A 20111222; IT PD20100392 A 20101223