EP 2687594 A1 20140122 - CULTURE METHOD, GROUP OF MATURE ADIPOCYTES, AND DRUG SCREENING METHOD
Title (en)
CULTURE METHOD, GROUP OF MATURE ADIPOCYTES, AND DRUG SCREENING METHOD
Title (de)
KULTURVERFAHREN, GRUPPE VON REIFEN ADIPOZYTEN UND ARZNEIMITTEL-SCREENING-VERFAHREN
Title (fr)
PROCÉDÉ DE CULTURE, GROUPE D'ADIPOCYTES MATURES, ET PROCÉDÉ DE CRIBLAGE DE MÉDICAMENTS
Publication
Application
Priority
- JP 2011057770 A 20110316
- JP 2012001872 W 20120316
Abstract (en)
In a culture method for adipocytes, seeded adipocytes are cultured while being allowed to adhere to a culture bottom surface (14) and walls (12) disposed perpendicularly to the culture bottom surface (14), without suspending the adipocytes in a culture solution, thereby obtaining mature adipocytes in which spherical lipid droplets having increased in size are generated within cells. This method uses a culture chamber (10) in which the walls (12) are formed perpendicular to the culture bottom surface (14). Spherical mature adipocytes containing spherical lipid droplets having increased in size within cells are cultured in a state where a cell or an aggregate of cells is allowed to adhere to two or more locations on the walls (12) and the culture bottom surface (14), thereby obtaining adipocytes having a shape similar to that of adipocytes in vivo.
IPC 8 full level
C12N 5/077 (2010.01); C12M 3/00 (2006.01); G01N 33/50 (2006.01)
CPC (source: EP KR US)
C12M 23/12 (2013.01 - EP KR US); C12M 23/20 (2013.01 - KR); C12M 23/22 (2013.01 - KR); C12M 25/06 (2013.01 - EP US); C12N 5/0653 (2013.01 - EP KR US); G01N 33/5044 (2013.01 - EP KR US); C12N 2501/33 (2013.01 - KR); C12N 2501/39 (2013.01 - KR); C12N 2503/02 (2013.01 - KR); C12N 2513/00 (2013.01 - EP KR US)
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
DOCDB simple family (publication)
EP 2687594 A1 20140122; EP 2687594 A4 20141112; CN 103608451 A 20140226; JP 6021802 B2 20161109; JP WO2012124353 A1 20140717; KR 20140009459 A 20140122; KR 20190002732 A 20190108; KR 20200021108 A 20200227; US 2014065659 A1 20140306; WO 2012124353 A1 20120920
DOCDB simple family (application)
EP 12757775 A 20120316; CN 201280013520 A 20120316; JP 2012001872 W 20120316; JP 2013504581 A 20120316; KR 20137026508 A 20120316; KR 20187037546 A 20120316; KR 20207005061 A 20120316; US 201214005508 A 20120316