EP 2692863 B1 20180620 - PCR PRIMERS FOR PREPARING SAMPLES FOR SINGLE NUCLEOTIDE POLYMORPHISM DETECTION COMPRISING ALLELE-SPECIFIC PCR AND ION EXCHANGE CHROMATOGRAPHIC DETECTION
Title (en)
PCR PRIMERS FOR PREPARING SAMPLES FOR SINGLE NUCLEOTIDE POLYMORPHISM DETECTION COMPRISING ALLELE-SPECIFIC PCR AND ION EXCHANGE CHROMATOGRAPHIC DETECTION
Title (de)
PCR-PRIMER ZUR HERSTELLUNG VON PROBEN ZUR ERKENNUNG VON EINZELNUKLEOTID-POLYMORPHISMEN MITTELS ALLELE-SPEZIFISCHER PCR UND IONENAUSTAUSCH-CHROMATOGRAPHIE-ANALYSE.
Title (fr)
AMORCES DE PCR POUR LA PREPARATION D'ECHANTILLONS POUR DETECTER DES POLYMORPHISMES MONONUCLEOTIDIQUES COMPRENANT UNE AMPLIFICATION PAR PCR SPECIFIQUE D'UN ALLELE ET ANALYSE DE LA DETECTION PAR CHROMATOGRAPHIE PAR ECHANGE D'IONS.
Publication
Application
Priority
- JP 2011080369 A 20110331
- JP 2012058701 W 20120330
Abstract (en)
[origin: EP2692863A1] An object of the present invention is to provide a sample nucleic acid for single nucleotide polymorphism detection which is for use in a simple method for quickly detecting a single nucleotide polymorphism, PCR primers for preparation of a sample for single nucleotide polymorphism detection, and a sample for single nucleotide polymorphism detection which is for use in ion exchange chromatography analysis. The present invention provides a sample nucleic acid for single nucleotide polymorphism detection having the following features: (a) a full chain length of 200 bp or less; (b) a sequence (a tag sequence) incompletely complementary to a template DNA at the 5' or 3' end; and (c) a 10 bp or less difference in chain length from a sample nucleic acid to be compared with to determine the presence of a single nucleotide polymorphism.
IPC 8 full level
G01N 30/88 (2006.01); C12N 15/00 (2006.01); C12Q 1/68 (2018.01); G01N 30/26 (2006.01)
CPC (source: EP KR US)
C12Q 1/6858 (2013.01 - EP KR US); C12Q 1/6881 (2013.01 - US); C12Q 2565/137 (2013.01 - KR); C12Q 2600/154 (2013.01 - KR)
Citation (examination)
EP 0507591 A2 19921007 - PERKIN ELMER CORP [US]
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
DOCDB simple family (publication)
EP 2692863 A1 20140205; EP 2692863 A4 20150225; EP 2692863 B1 20180620; CN 103443274 A 20131211; JP WO2012133834 A1 20140728; KR 20140026426 A 20140305; US 2014349284 A1 20141127; WO 2012133834 A1 20121004
DOCDB simple family (application)
EP 12765405 A 20120330; CN 201280014783 A 20120330; JP 2012058701 W 20120330; JP 2013507820 A 20120330; KR 20137027899 A 20120330; US 201214008770 A 20120330