EP 2694672 A1 20140212 - METHOD FOR THE LYSIS OF CELLS AND PCR AMPLIFICATION
Title (en)
METHOD FOR THE LYSIS OF CELLS AND PCR AMPLIFICATION
Title (de)
VERFAHREN ZUR LYSE VON ZELLEN UND PCR-AMPLIFIKATION
Title (fr)
PROCÉDÉ POUR LA LYSE DE CELLULES ET L'AMPLIFICATION ACP
Publication
Application
Priority
- DE 102011007035 A 20110408
- EP 2012052072 W 20120208
Abstract (en)
[origin: WO2012136400A1] The invention relates to a LOC (lab-on-a-chip) (10) for the lysis of cells and PCR amplification, having a substrate (11), a fluidic network (12) in the substrate (11), an externally temperature-controlled filter (16) on the substrate (11), an externally temperature-controlled PCR chamber (17) on the substrate (11), and optionally an array (18) on the substrate (11), wherein the filter (16), the PCR chamber (17) and the array (43) are connected to the fluidic network (12). A method according to the invention for the lysis of cells and PCR amplification comprises the method steps of: a) positioning the cells on a filter (16); b) dyeing the cells; c) adding a PCR treatment solution; d) photolysis of the cells; and e) amplifying DNA of the cells by means of PCR; wherein method steps a) and b) as well as c) and d) can occur in any sequence.
IPC 8 full level
C12Q 1/68 (2006.01)
CPC (source: EP)
B01L 3/502753 (2013.01); B01L 7/52 (2013.01); B01L 2200/10 (2013.01); B01L 2300/0681 (2013.01); B01L 2300/0816 (2013.01); B01L 2300/0867 (2013.01); B01L 2300/0877 (2013.01)
Citation (search report)
See references of WO 2012136400A1
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
DOCDB simple family (publication)
DE 102011007035 A1 20121011; EP 2694672 A1 20140212; EP 2694672 B1 20180912; WO 2012136400 A1 20121011
DOCDB simple family (application)
DE 102011007035 A 20110408; EP 12704033 A 20120208; EP 2012052072 W 20120208