EP 2694680 A4 20140910 - METHODS AND KITS FOR DETECTING CELL-FREE PATHOGEN-SPECIFIC NUCLEIC ACIDS
Title (en)
METHODS AND KITS FOR DETECTING CELL-FREE PATHOGEN-SPECIFIC NUCLEIC ACIDS
Title (de)
VERFAHREN UND KITS ZUM NACHWEIS ZELLFREIER KRANKHEITSERREGERSPEZIFISCHER NUKLEINSÄUREN
Title (fr)
PROCÉDÉS ET NÉCESSAIRES POUR DÉTECTION D'ACIDES NUCLÉIQUES SPÉCIFIQUES DE PATHOGÈNE, EXEMPTS DE CELLULES
Publication
Application
Priority
- US 201161470774 P 20110401
- US 2012031814 W 20120402
Abstract (en)
[origin: WO2012135815A2] The present invention relates to a method for detecting a target nucleic acid derived from a pathogen in a subject. The method comprises (a) amplifying the nucleic acid sequence of the target nucleic acid, which is obtained from a cell-free fraction of a blood sample from the subject, to produce a double stranded DNA is produced, and (b) detecting the double stranded DNA. The presence of the double stranded DNA indicates the presence of the target nucleic acid in the subject. Also provided are kits for detecting a target nucleic acid derived from a pathogen in a subject.
IPC 8 full level
C12Q 1/68 (2006.01); C12N 15/11 (2006.01); C12Q 1/04 (2006.01)
CPC (source: CN EP RU US)
C12Q 1/689 (2013.01 - CN EP RU US)
Citation (search report)
- [XI] STADEN VAN M ET AL: "Detection of Mycobacterium tuberculosis in serum samples using the polymerase chain reaction", JOURNAL OF INFECTION, ACADEMIC PRESS, LONDON, GB, vol. 36, no. 3, 1 May 1998 (1998-05-01), pages 273 - 277, XP002645865, ISSN: 0163-4453
- [A] MARIA L.R. ROSSETTI ET AL: "Improvement of Mycobacterium tuberculosis detection in clinical samples using DNA purified by glass matrix", JOURNAL OF MICROBIOLOGICAL METHODS, vol. 28, no. 2, 1 February 1997 (1997-02-01), pages 139 - 146, XP055132543, ISSN: 0167-7012, DOI: 10.1016/S0167-7012(97)00978-0
- See references of WO 2012135815A2
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
DOCDB simple family (publication)
WO 2012135815 A2 20121004; WO 2012135815 A3 20121227; AU 2012236109 A1 20131121; AU 2017203071 A1 20170601; AU 2019204844 A1 20190725; CN 103814139 A 20140521; CN 103814139 B 20181113; CN 109280713 A 20190129; EP 2694680 A2 20140212; EP 2694680 A4 20140910; JP 2014510538 A 20140501; JP 2018117645 A 20180802; JP 6430826 B2 20181128; RU 2013148806 A 20150510; RU 2644672 C2 20180213; US 2014147851 A1 20140529; US 2017369932 A1 20171228
DOCDB simple family (application)
US 2012031814 W 20120402; AU 2012236109 A 20120402; AU 2017203071 A 20170509; AU 2019204844 A 20190705; CN 201280026842 A 20120402; CN 201811203829 A 20120402; EP 12765867 A 20120402; JP 2014502902 A 20120402; JP 2018091537 A 20180510; RU 2013148806 A 20120402; US 201214009199 A 20120402; US 201715664074 A 20170731