EP 2697396 B1 20191113 - METHOD AND APPARATUS FOR HIGH ACCURACY GENOMIC ANALYSIS PLATFORM UTILIZING HYBRIDIZATION AND ENHANCED DISSOCIATION
Title (en)
METHOD AND APPARATUS FOR HIGH ACCURACY GENOMIC ANALYSIS PLATFORM UTILIZING HYBRIDIZATION AND ENHANCED DISSOCIATION
Title (de)
VERFAHREN UND VORRICHTUNG FÜR EINE HOCHPRÄZISE GENOMANALYSEPLATTFORM MIT HYBRIDISIERUNG UND VERBESSERTER DISSOZIATION
Title (fr)
PROCÉDÉ ET APPAREIL POUR UNE PLATEFORME D'ANALYSE GÉNOMIQUE DE GRANDE PRÉCISION UTILISANT L'HYBRIDATION ET LA DISSOCIATION AMÉLIORÉE
Publication
Application
Priority
- US 201161474727 P 20110412
- US 2012033406 W 20120412
Abstract (en)
[origin: WO2012142346A1] Methods and an apparatus are provided for capture/enrichment, gene expression profiling and targeted sequencing. Provided are methods for improving accuracy and stringency of micro arrays and/or other genomic analysis methods relying on nucleic acid hybridization and melting curve analysis by controlling surface chemistry. This method comprises producing a positively charged surface or surface coating, on the surface of microarray slides or other types of surfaces simlady purposed, such as micro beads, which enhances melting curve analysis to the point of allowing detection or differentiation of small changes in sequences between nucleic acid binding partners. Also provided is an improved micro array reader machine, to collect melting curve data on microarray slides. The accuracy or resolution of melting curve analysis was to be sufficient to distinguish between the melting of perfect matched dsDNA and dsDNA with the smallest possible change in sequence, a one base pair mismatch.
IPC 8 full level
C12Q 1/6837 (2018.01); C12Q 1/6834 (2018.01); C12Q 1/6874 (2018.01)
CPC (source: CN EP US)
C12Q 1/6834 (2013.01 - EP US); C12Q 1/6837 (2013.01 - CN EP US); C12Q 1/6874 (2013.01 - CN EP US); C12Q 2527/107 (2013.01 - EP US); C12Q 2527/125 (2013.01 - EP US)
Citation (examination)
- WO 2010038170 A1 20100408 - KONINKL PHILIPS ELECTRONICS NV [NL], et al
- ARNOLD VAINRUB ET AL: "Surface electrostatic effects in oligonucleotide microarrays: Control and optimization of binding thermodynamics", BIOPOLYMERS, 1 February 2003 (2003-02-01), New York, pages 265 - 270, XP055133705, Retrieved from the Internet <URL:http://onlinelibrary.wiley.com/doi/10.1002/bip.10271/abstract> DOI: 10.1002/bip.10271
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
DOCDB simple family (publication)
WO 2012142346 A1 20121018; CN 103857803 A 20140611; EP 2697396 A1 20140219; EP 2697396 A4 20141203; EP 2697396 B1 20191113; US 2012289421 A1 20121115; US 9856523 B2 20180102
DOCDB simple family (application)
US 2012033406 W 20120412; CN 201280028732 A 20120412; EP 12771263 A 20120412; US 201213445873 A 20120412