EP 2726501 B1 20180822 - SULFATION OF WNT PATHWAY PROTEINS
Title (en)
SULFATION OF WNT PATHWAY PROTEINS
Title (de)
SULFATIERUNG VON WNT-SIGNALWEGPROTEINEN
Title (fr)
SULFATATION DE PROTÉINES DE LA VOIE WNT
Publication
Application
Priority
- US 201113170634 A 20110628
- US 2012043451 W 20120621
Abstract (en)
[origin: US2012071399A1] Provided is a composition comprising a peptide comprising amino acids and/or amino acid analogs comprising a continuous sequence of a sclerostin fragment comprising Tyr43 or Tyr213. Also provided is a composition comprising a peptide comprising less than about 75 amino acids and/or amino acid analogs including an amino acid or amino acid analog capable of being sulfated, where the composition is capable of inhibiting sclerostin binding to an LRP. Further provided is a composition comprising a peptide comprising less than about 75 amino acids and/or amino acid analogs including an amino acid or amino acid analog capable of being post-translationally sulfated, where the composition is capable of inhibiting binding of a protein ligand comprising a sulfation site to its binding partner. Additionally provided is a method of enhancing a Wnt signaling pathway comprising contacting an LRP5/6 receptor in the Wnt signaling pathway with either of the above-described compositions that comprise a sequence of a sclerostin fragment or is capable of inhibiting sclerostin binding to an LRP, where the tyrosine or tyrosine analog is not sulfated, in a manner sufficient to enhance the Wnt signaling pathway. Further provided is a method of treating a subject having a disease exacerbated by inhibition of a Wnt signaling pathway comprising administering either of the above-described compositions that comprise a sequence of a sclerostin fragment or is capable of inhibiting sclerostin binding to an LRP, where the tyrosine or tyrosine analog is not sulfated, to the subject in a manner sufficient to reduce the inhibition of the Wnt signaling pathway. Also, a method of inhibiting binding of a protein ligand comprising a sulfation site to its binding partner is provided. The method comprises adding the above-described composition that is capable of inhibiting binding of a protein ligand to its binding partner to the protein ligand and its binding partner in a manner sufficient to inhibit binding of the protein ligand to its binding partner.
IPC 8 full level
C07K 14/705 (2006.01); A61K 38/08 (2019.01); A61K 38/10 (2006.01); A61K 38/17 (2006.01); C07K 14/47 (2006.01); C07K 14/51 (2006.01); C07K 14/71 (2006.01)
CPC (source: EP US)
A61K 38/08 (2013.01 - EP US); A61K 38/10 (2013.01 - EP US); A61K 38/1709 (2013.01 - EP US); A61P 3/04 (2017.12 - EP); A61P 3/10 (2017.12 - EP); A61P 9/00 (2017.12 - EP); A61P 9/10 (2017.12 - EP); A61P 9/12 (2017.12 - EP); A61P 13/12 (2017.12 - EP); A61P 17/14 (2017.12 - EP); A61P 19/02 (2017.12 - EP); A61P 19/06 (2017.12 - EP); A61P 19/08 (2017.12 - EP); A61P 19/10 (2017.12 - EP); A61P 35/00 (2017.12 - EP); C07K 14/47 (2013.01 - EP US); C07K 14/51 (2013.01 - EP US); C07K 14/705 (2013.01 - EP US); C07K 14/71 (2013.01 - EP US)
Citation (examination)
- WO 2011156252 A2 20111215 - ENZO BIOCHEM INC [US], et al
- WO 2006119107 A2 20061109 - UCB SA [GB], et al
- THEODORE A. CRAIG ET AL: "Production and Characterization of Monoclonal Antibodies to Human Sclerostin", HYBRIDOMA, vol. 28, no. 5, 1 October 2009 (2009-10-01), pages 377 - 381, XP055008009, ISSN: 1554-0014, DOI: 10.1089/hyb.2009.0036
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
DOCDB simple family (publication)
US 2012071399 A1 20120322; US 9403882 B2 20160802; CA 2838290 A1 20130103; CA 2838290 C 20200901; EP 2726501 A1 20140507; EP 2726501 B1 20180822; EP 3428183 A1 20190116; EP 3428183 B1 20240320; EP 3428183 B8 20240424; US 10328120 B2 20190625; US 2016317616 A1 20161103; WO 2013003178 A1 20130103
DOCDB simple family (application)
US 201113170634 A 20110628; CA 2838290 A 20120621; EP 12730347 A 20120621; EP 18173173 A 20120621; US 2012043451 W 20120621; US 201615195017 A 20160628