EP 2739643 A1 20140611 - USE OF FMN-BINDING FLUORESCENCE PROTEINS (FBFP) AS NEW TYPES OF SECRETION MARKERS
Title (en)
USE OF FMN-BINDING FLUORESCENCE PROTEINS (FBFP) AS NEW TYPES OF SECRETION MARKERS
Title (de)
VERWENDUNG VON FMN-BINDENDEN FLUORESZENZPROTEINEN (FBFP) ALS NEUARTIGE SEKRETIONSMARKER
Title (fr)
UTILISATION DE PROTÉINES FLUORESCENTES LIANT LE FMN (FBFP) COMME NOUVEAU MARQUEUR DE SÉCRÉTION
Publication
Application
Priority
- DE 102011118025 A 20110805
- EP 2012064776 W 20120727
Abstract (en)
[origin: WO2013020831A1] To investigate protein-protein interactions, protein foldings and protein localization and also in the secretion of proteins, in vivo reporter proteins are used in biotechnology and in basic research. In order to be able to utilize fluorescence reporters as markers for secretion processes, FMN-binding fluorescence proteins (FbFP) have been developed by us for the first time. The new fluorescence markers can be expressed like GFP in various bacteria. The binding of the chromophore FMN produces a cyan-green fluorescent protein which can be detected in vivo using all customary spectroscopic and microscopic methods. In contrast to GFP, this protein can also surprisingly be secreted via the Sec route and be converted to the fluorescence-active state in the periplasma.
IPC 8 full level
C07K 14/195 (2006.01)
CPC (source: EP US)
C07K 14/195 (2013.01 - EP US); C07K 14/245 (2013.01 - US); C12Q 1/68 (2013.01 - US); G01N 33/52 (2013.01 - US)
Citation (search report)
See references of WO 2013020831A1
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
DOCDB simple family (publication)
WO 2013020831 A1 20130214; EP 2739643 A1 20140611; US 2014227698 A1 20140814
DOCDB simple family (application)
EP 2012064776 W 20120727; EP 12740173 A 20120727; US 201214237282 A 20120727