EP 2756064 A2 20140723 - METHOD FOR ADAPTING A HYDROLYTIC ENZYME TO A COMPONENT THAT STABILIZES THE HYDROLYTIC ENZYME
Title (en)
METHOD FOR ADAPTING A HYDROLYTIC ENZYME TO A COMPONENT THAT STABILIZES THE HYDROLYTIC ENZYME
Title (de)
VERFAHREN ZUR ANPASSUNG EINES HYDROLYTISCHEN ENZYMS AN EINE DAS HYDROLYTISCHE ENZYM STABILISIERENDE KOMPONENTE
Title (fr)
PROCÉDÉ D'ADAPTATION D'UNE ENZYME HYDROLYTIQUE À UN CONSTITUANT STABILISANT LADITE ENZYME HYDROLYTIQUE
Publication
Application
Priority
- DE 102011118027 A 20110912
- EP 2012066237 W 20120821
Abstract (en)
[origin: WO2013037609A2] The stabilization of a hydrolytic enzyme in a liquid preparation is to be improved through a component that stabilizes the hydrolytic enzyme. This is accomplished by a method for adapting a hydrolytic enzyme to a component that stabilizes the hydrolytic enzyme, comprising the following method steps: a) providing a hydrolytic enzyme (starting enzyme) and a component that stabilizes the hydrolytic enzyme and that comprises a reversible inhibitor of the hydrolytic enzyme; b) changing the amino acid sequence of the hydrolytic enzyme in at least one position, by substitution, deletion or insertion; c) determining the relative activity of the hydrolytic enzyme from step b) and the relative activity of the starting enzyme in a liquid preparation; d) selecting the hydrolytic enzyme that has a reduced relative activity by comparison with the relative activity of the starting enzyme.
IPC 8 full level
C11D 3/386 (2006.01); C12N 9/54 (2006.01)
CPC (source: EP US)
C11D 3/38663 (2013.01 - EP US); C11D 3/38681 (2013.01 - EP US); C12N 9/54 (2013.01 - EP US); C12N 9/96 (2013.01 - EP US); C12Q 1/37 (2013.01 - US)
Citation (search report)
See references of WO 2013037609A2
Citation (examination)
WO 2008002472 A2 20080103 - DANISCO US INC GENENCOR DIV [US], et al
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
DOCDB simple family (publication)
DE 102011118027 A1 20130314; EP 2756064 A2 20140723; EP 3067411 A1 20160914; US 2014186868 A1 20140703; US 9695461 B2 20170704; WO 2013037609 A2 20130321; WO 2013037609 A3 20130530
DOCDB simple family (application)
DE 102011118027 A 20110912; EP 12755969 A 20120821; EP 16164786 A 20120821; EP 2012066237 W 20120821; US 201414199569 A 20140306