EP 2780479 A4 20150408 - QUANTITATIVE, HIGHLY MULTIPLEXED DETECTION OF NUCLEIC ACIDS
Title (en)
QUANTITATIVE, HIGHLY MULTIPLEXED DETECTION OF NUCLEIC ACIDS
Title (de)
QUANTITATIVER HOCHMULTIPLEXIERTER NACHWEIS VON NUKLEINSÄUREN
Title (fr)
DÉTECTION QUANTITATIVE, FORTEMENT MULTIPLEXÉE D'ACIDES NUCLÉIQUES
Publication
Application
Priority
- US 201161561198 P 20111117
- US 2012025699 W 20120217
- US 201213399872 A 20120217
- US 2012051236 W 20120816
Abstract (en)
[origin: WO2013074163A1] This invention provides methods of detecting and quantifying target nucleic acids in samples in multiplexed single chamber reactions. Consumables incorporating chambers optimized to reduce signal background proximal to high efficiency arrays are provided, as well as methods of use. Devices and systems configured to use the consumables to practice the methods are a feature of the invention.
IPC 8 full level
C12Q 1/68 (2006.01); C12N 15/11 (2006.01)
CPC (source: CN EP KR)
C12Q 1/6813 (2013.01 - KR); C12Q 1/6837 (2013.01 - CN EP); C12Q 1/6844 (2013.01 - KR)
C-Set (source: CN EP)
CN
C12Q 1/6837 + C12Q 2531/113 + C12Q 2563/107 + C12Q 2565/101
EP
C12Q 1/6837 + C12Q 2525/15 + C12Q 2525/204 + C12Q 2527/107 + C12Q 2531/113 + C12Q 2563/107 + C12Q 2565/1015 + C12Q 2565/601
Citation (search report)
- [Y] US 2008241838 A1 20081002 - SCABOO KRISTIAN [US], et al
- [A] US 6350580 B1 20020226 - SORGE JOSEPH A [US]
- [A] EP 0601834 A1 19940615 - WISCONSIN ALUMNI RES FOUND [US]
- [Y] BERNARD JUSKOWIAK: "Nucleic acid-based fluorescent probes and their analytical potential", ANALYTICAL AND BIOANALYTICAL CHEMISTRY, SPRINGER, BERLIN, DE, vol. 399, no. 9, 29 October 2010 (2010-10-29), pages 3157 - 3176, XP019885328, ISSN: 1618-2650, DOI: 10.1007/S00216-010-4304-5
- [A] MATSUBARA Y ET AL: "Microchamber array based DNA quantification and specific sequence detection from a single copy via PCR in nanoliter volumes", BIOSENSORS AND BIOELECTRONICS, ELSEVIER BV, NL, vol. 20, no. 8, 15 February 2005 (2005-02-15), pages 1482 - 1490, XP027619503, ISSN: 0956-5663, [retrieved on 20050215]
- [A] SHALON D ET AL: "A DNA MICROARRAY SYSTEM FOR ANALYZING COMPLEX DNA SAMPLES USING TWO-COLOR FLUORESCENT PROBE HYBRIDIZATION", GENOME RESEARCH, COLD SPRING HARBOR LABORATORY PRESS, WOODBURY, NY, US, vol. 6, no. 7, 1 July 1996 (1996-07-01), pages 639 - 645, XP009007958, ISSN: 1088-9051
- See references of WO 2013074163A1
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
DOCDB simple family (publication)
WO 2013074163 A1 20130523; AU 2012337389 A1 20140619; BR 112014011937 A2 20170530; CA 2855953 A1 20130523; CN 104093857 A 20141008; EP 2780479 A1 20140924; EP 2780479 A4 20150408; HK 1199746 A1 20150717; IL 232586 A0 20140630; JP 2014533502 A 20141215; KR 20140094007 A 20140729; MX 2014005912 A 20140904; MX 338076 B 20160401; SG 11201402341Q A 20140627
DOCDB simple family (application)
US 2012051236 W 20120816; AU 2012337389 A 20120816; BR 112014011937 A 20120816; CA 2855953 A 20120816; CN 201280067273 A 20120816; EP 12849697 A 20120816; HK 15100214 A 20150108; IL 23258614 A 20140513; JP 2014542300 A 20120816; KR 20147016411 A 20120816; MX 2014005912 A 20120816; SG 11201402341Q A 20120816