EP 2788478 B1 20170823 - MULTIPLEX IMMUNO SCREENING ASSAY
Title (en)
MULTIPLEX IMMUNO SCREENING ASSAY
Title (de)
MULTIPLEX-IMMUNOSCREENING-TEST
Title (fr)
IMMUNOESSAI DE DÉPISTAGE MULTIPLEX
Publication
Application
Priority
- US 201261642924 P 20120504
- EP 2011072387 W 20111209
- EP 2012074986 W 20121210
Abstract (en)
[origin: WO2013164476A1] The present invention provides kits and assay methods for the early detection of pathogens, precise identification of the etiologic agent, and improved disease surveillance. More specifically, the present invention discloses an immunoassay leading to the rapid and simultaneous detection of antibodies to a wide range of infectious pathogens in biological fluids of infected patients. This immunoassay involves the covalent and oriented coupling of fusion proteins comprising an AGT enzyme and a viral antigen on an identifiable solid support (e.g. fluorescent microspheres), said support being previously coated with an AGT substrate. This coupling is mediated by the irreversible reaction of the AGT enzyme on its substrate. The thus obtained antigen-coupled microspheres show enhanced capture of specific antibodies as compared to antigen-coupled microspheres produced by standard amine coupling procedures. The methods of the invention possess the ability to multiplex, minimize the amount of biological sample, and have enhanced sensitivity and specificity toward target antibodies as compared with classical ELISA or Radio-Immunoprecipitation assays.
IPC 8 full level
C12N 9/10 (2006.01); C12N 15/62 (2006.01); G01N 33/543 (2006.01); G01N 33/564 (2006.01)
CPC (source: CN EP GB US)
G01N 33/543 (2013.01 - EP US); G01N 33/54306 (2013.01 - US); G01N 33/54313 (2013.01 - EP US); G01N 33/54353 (2013.01 - EP US); G01N 33/564 (2013.01 - CN EP US); G01N 33/56983 (2013.01 - EP US); G01N 33/6845 (2013.01 - CN EP US); G02B 5/1814 (2013.01 - GB); G02B 6/0016 (2013.01 - US); G02B 6/0038 (2013.01 - US); G02B 27/0081 (2013.01 - CN EP US); G02B 27/0172 (2013.01 - CN EP US); G02B 27/4272 (2013.01 - EP GB US); G06T 19/006 (2013.01 - GB); G01N 2333/18 (2013.01 - EP US); G01N 2333/185 (2013.01 - US); G01N 2469/20 (2013.01 - EP US); G02B 27/4272 (2013.01 - CN); G02B 2027/0125 (2013.01 - US); G02B 2027/0174 (2013.01 - CN EP US); Y02A 50/30 (2017.12 - US)
Citation (examination)
- WO 2006107921 A2 20061012 - ALLGERGAN INC [US], et al
- WO 2008036060 A2 20080327 - ALLERGAN INC [US], et al
- SINEM ENGIN ET AL: "Supporting Information: Benzylguanine Thiol Self-Assembled Monolayers for the Immobilization of SNAP-tag Proteins on Microcontact-Printed Surface Structures", LANGMUIR LETTER, 4 June 2010 (2010-06-04), pages 1 - 6, XP055308163, Retrieved from the Internet <URL:http://pubs.acs.org/doi/suppl/10.1021/la904829y> [retrieved on 20161006]
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
DOCDB simple family (publication)
WO 2013164476 A1 20131107; AU 2012350229 A1 20140717; AU 2012350229 B2 20180322; AU 2013255744 A1 20141113; BR 112014013745 A2 20171212; BR 112014013745 B1 20220614; BR 112014027465 A2 20170627; BR 112014027465 A8 20210629; BR 112014027465 B1 20220712; CA 2857998 A1 20130613; CA 2857998 C 20210119; CA 2872127 A1 20131107; CN 104114697 A 20141022; CN 104704117 A 20150610; CN 104704117 B 20180803; DK 2788478 T3 20171009; EP 2788478 A2 20141015; EP 2788478 B1 20170823; EP 2844748 A1 20150311; EP 2844748 B1 20180711; ES 2648899 T3 20180108; JP 2015500989 A 20150108; JP 2015517649 A 20150622; JP 6215223 B2 20171018; JP 6351571 B2 20180704; KR 102007061 B1 20190802; KR 102049623 B1 20191128; KR 20140113937 A 20140925; KR 20150016941 A 20150213; MX 2014006870 A 20141125; MX 2014013417 A 20141126; MX 349562 B 20170802; MX 357087 B 20180625; NO 2788478 T3 20180120; NZ 701380 A 20160331; SG 11201403010T A 20140730; SG 11201407167R A 20141127; US 10119967 B2 20181106; US 10197562 B2 20190205; US 10209248 B2 20190219; US 10352930 B2 20190716; US 2014274762 A1 20140918; US 2015099656 A1 20150409; US 2017276672 A1 20170928; US 2017336412 A1 20171123; US 2018038852 A1 20180208; US 9638692 B2 20170502; WO 2013083847 A2 20130613; WO 2013083847 A3 20130801
DOCDB simple family (application)
EP 2013059312 W 20130503; AU 2012350229 A 20121210; AU 2013255744 A 20130503; BR 112014013745 A 20121210; BR 112014027465 A 20130503; CA 2857998 A 20121210; CA 2872127 A 20130503; CN 201280069229 A 20121210; CN 201380035238 A 20130503; DK 12798308 T 20121210; EP 12798308 A 20121210; EP 13723051 A 20130503; EP 2012074986 W 20121210; ES 12798308 T 20121210; JP 2014545306 A 20121210; JP 2015509458 A 20130503; KR 20147018819 A 20121210; KR 20147031586 A 20130503; MX 2014006870 A 20121210; MX 2014013417 A 20130503; NO 12798308 A 20121210; NZ 70138013 A 20130503; SG 11201403010T A 20121210; SG 11201407167R A 20130503; US 201213883339 A 20121210; US 201314396841 A 20130503; US 201715480451 A 20170406; US 201715653949 A 20170719; US 201715654272 A 20170719