Global Patent Index - EP 2792744 A4

EP 2792744 A4 20150819 - GENE TARGETING VECTOR, AND METHOD FOR USING SAME

Title (en)

GENE TARGETING VECTOR, AND METHOD FOR USING SAME

Title (de)

GENTARGETINGVEKTOR UND VERWENDUNGSVERFAHREN DAFÜR

Title (fr)

VECTEUR DE CIBLAGE GÉNIQUE ET SON PROCÉDÉ D'UTILISATION

Publication

EP 2792744 A4 20150819 (EN)

Application

EP 12857876 A 20121212

Priority

  • JP 2011272072 A 20111213
  • JP 2012082160 W 20121212

Abstract (en)

[origin: EP2792744A1] Provided is a gene targeting vector that enables highly efficient gene targeting. The gene targeting vector has a structure comprising a positive selection marker flanked by a DNA homologous to a 5'-upstream region of a target site and a DNA homologous to a 3'-downstream region of the target site, wherein a splice acceptor site and a DNA sequence allowing for bicistronic expression are added 5'-upstream of the positive selection marker, and another splice acceptor site is also added 5'-upstream of the DNA homologous to the 5'-upstream region of the target site.

IPC 8 full level

C12N 15/09 (2006.01); C12N 1/15 (2006.01); C12N 1/19 (2006.01); C12N 1/21 (2006.01); C12N 5/10 (2006.01)

CPC (source: EP US)

C12N 15/09 (2013.01 - US); C12N 15/85 (2013.01 - US); C12N 15/8509 (2013.01 - EP US); C12N 15/907 (2013.01 - EP US); C12N 15/63 (2013.01 - EP US); C12N 2830/50 (2013.01 - EP US); C12N 2840/203 (2013.01 - EP US); C12N 2840/44 (2013.01 - EP US)

Citation (search report)

  • [XY] WO 2004042007 A2 20040521 - UNIV IOWA RES FOUND [US], et al
  • [XY] WO 03044203 A2 20030530 - CHAPPUIS-FLAMENT SOPHIE [FR]
  • [XY] WO 2004072251 A2 20040826 - WISCONSIN ALUMNI RES FOUND [US]
  • [XY] NORITAKA ADACHI ET AL: "Gene targeting using the human Nalm-6 pre-B cell line", BIOSCIENCE TRENDS, 1 October 2008 (2008-10-01), Japan, pages 169, XP055201791, Retrieved from the Internet <URL:http://www.ncbi.nlm.nih.gov/pubmed/20103924>
  • [XY] ONO T ET AL: "Generation of tetracycline-inducible conditional gene knockout cells in a human Nalm-6 cell line", JOURNAL OF BIOTECHNOLOGY, ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, NL, vol. 141, no. 1-2, 20 April 2009 (2009-04-20), pages 1 - 7, XP026049495, ISSN: 0168-1656, [retrieved on 20090415], DOI: 10.1016/J.JBIOTEC.2008.12.010
  • [XY] GUO YI ET AL: "Rapid Construction of Rabbit HPRT Promoterless Gene-targeting Vector with PCR Product", INTERNET CITATION, 2009, pages 86 - 92, XP002733577, Retrieved from the Internet <URL:http://211.144.119.130:8080/jweb/EN/abstract/abstract3466.shtml> [retrieved on 20141210]
  • See references of WO 2013089123A1

Designated contracting state (EPC)

AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

DOCDB simple family (publication)

EP 2792744 A1 20141022; EP 2792744 A4 20150819; EP 2792744 B1 20170726; CN 104053773 A 20140917; JP 6037290 B2 20161207; JP WO2013089123 A1 20150427; US 2014335621 A1 20141113; US 9303272 B2 20160405; WO 2013089123 A1 20130620

DOCDB simple family (application)

EP 12857876 A 20121212; CN 201280066047 A 20121212; JP 2012082160 W 20121212; JP 2013549280 A 20121212; US 201214364922 A 20121212