EP 2817614 A1 20141231 - METHOD FOR NORMALISING THE LUMINESCENCE EMITTED BY A MEASURING MEDIUM
Title (en)
METHOD FOR NORMALISING THE LUMINESCENCE EMITTED BY A MEASURING MEDIUM
Title (de)
VERFAHREN ZUR NORMALISIERUNG DER VON EINEM MESSMEDIUM EMITTIERTEN LUMINESZENZ
Title (fr)
PROCEDE DE NORMALISATION DE LA LUMINESCENCE EMISE PAR UN MILIEU DE MESURE.
Publication
Application
Priority
FR 2012050370 W 20120222
Abstract (en)
[origin: WO2013124544A1] The subject matter of the invention is a method for measuring the luminescence of a long-life fluorescent compound present in a measuring medium, said medium containing a biological sample, characterised in that it comprises the following steps: a) introduction of a long-life fluorescent compound into the measuring medium, b) introduction, into the measuring medium, of a fluorescent marking agent, the absorption spectrum of which allows the excitation thereof at the same wavelength as that used to excite the long-life fluorescent compound, wherein said emission spectrum allows the measurement of the luminescence thereof at the same wavelength as that used to measure the luminescence of the long-life fluorescent compound, c) excitation of the measuring medium with a wavelength corresponding to an absorption peak of the long-life fluorescent compound, d) measurement of the luminescence emitted by the measuring medium immediately after excitation of said medium, mainly corresponding to the luminescence of the marking agent, and for a period of 5 ns to 45 ps, at a wavelength corresponding to an emission peak of the long-life fluorescent compound, e) time-resolved measurement of the luminescence emitted by the measuring medium at the same wavelength as that used in step d), after a delay of 20 to 200 ps following the excitation of the measuring medium and for a period of 200 to 1000 ps, said luminescence mainly corresponding to that of the long-life fluorescent compound, f) calculation of a normalised luminescence signal corresponding to the ratio: (signal obtained in step e) / (signal obtained in step d).
IPC 8 full level
G01N 21/64 (2006.01); G01N 33/542 (2006.01); G01N 33/58 (2006.01); G16B 99/00 (2019.01)
CPC (source: EP US)
G01N 21/64 (2013.01 - EP US); G01N 21/6408 (2013.01 - EP US); G01N 21/6428 (2013.01 - EP US); G01N 33/542 (2013.01 - EP US); G01N 33/58 (2013.01 - EP US); G01N 33/582 (2013.01 - US); G16B 99/00 (2019.01 - EP); G01N 21/274 (2013.01 - EP US); G01N 2021/6413 (2013.01 - US); G01N 2021/6432 (2013.01 - US); G01N 2021/6439 (2013.01 - US); G01N 2021/6441 (2013.01 - EP US); G01N 2201/06113 (2013.01 - US); G16B 99/00 (2019.01 - US)
Citation (search report)
See references of WO 2013124544A1
Citation (examination)
N. GABORIT ET AL: "Time-resolved Fluorescence Resonance Energy Transfer (TR-FRET) to Analyze the Disruption of EGFR/HER2 Dimers: A NEW METHOD TO EVALUATE THE EFFICIENCY OF TARGETED THERAPY USING MONOCLONAL ANTIBODIES", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 286, no. 13, 1 April 2011 (2011-04-01), pages 11337 - 11345, XP055021021, ISSN: 0021-9258, DOI: 10.1074/jbc.M111.223503
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
Designated extension state (EPC)
BA ME
DOCDB simple family (publication)
WO 2013124544 A1 20130829; EP 2817614 A1 20141231; US 2015185150 A1 20150702
DOCDB simple family (application)
FR 2012050370 W 20120222; EP 12710984 A 20120222; US 201214380259 A 20120222