EP 2821498 B1 20181212 - METHOD FOR ISOLATING UNDIFFERENTIATED STEM CELLS
Title (en)
METHOD FOR ISOLATING UNDIFFERENTIATED STEM CELLS
Title (de)
VERFAHREN ZUR ISOLIERUNG UNDIFFERENZIERTER STAMMZELLEN
Title (fr)
PROCÉDÉ D'ISOLEMENT DE CELLULES SOUCHES NON DIFFÉRENCIÉES
Publication
Application
Priority
- JP 2012041418 A 20120228
- JP 2012267679 A 20121206
- JP 2013001160 W 20130227
Abstract (en)
[origin: EP2821498A1] Provided are a method for accurately evaluating the differentiation status of stem cells by selectively staining only stem cells in an undifferentiated state, and a method for positively isolating only stem cells in an undifferentiated state. Specifically provided is a method for determining differentiation of a cell comprising a step of contacting a test cell with a probe comprising protein (A) or (B) below and a step of detecting the presence of binding of the probe to the test cell. The method for determining differentiation of a cell is capable of detecting the presence or absence of an undifferentiated stem cell in test cells by using a probe that specifically reacts with undifferentiated stem cells and detecting the presence of bonding to the test cell. (A) A protein comprising an amino acid sequence shown in SEQ ID NO: 1 and recognizing a sugar chain structure of "Fuc±1-2Gal²1-3GlcNAc" and/or "Fuc±1-2Gal²1-3GalNAc;" and (B) a protein comprising an amino acid sequence showing 80% or more similarity to the amino acid sequence shown in SEQ ID NO: 1 and recognizing a sugar chain structure of "Fuc±1-2Gal²1-3GlcNAc" and/or "Fuc±1-2Gal²1-3GalNAc."
IPC 8 full level
C12Q 1/02 (2006.01); C12M 1/00 (2006.01); C12M 1/34 (2006.01); C12N 5/10 (2006.01); G01N 33/569 (2006.01)
CPC (source: EP US)
G01N 33/56966 (2013.01 - EP US)
Citation (examination)
- CHUNG YOUNG ET AL: "Human embryonic stem cell lines generated without embryo destruction", CELL STEM CELL, vol. 2, no. 2, February 2008 (2008-02-01), pages 113 - 117, ISSN: 1934-5909
- SHARON NATHAN ET AL: "History of lectins: from hemagglutinins to biological recognition molecules", GLYCOBIOLOGY, vol. 14, no. 11, November 2004 (2004-11-01), pages 53R - 62R, ISSN: 0959-6658
- FU LEI-LEI ET AL: "Plant lectins: Targeting programmed cell death pathways as antitumor agents", INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, vol. 43, no. 10, October 2011 (2011-10-01), pages 1442 - 1449, ISSN: 1357-2725(print)
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
DOCDB simple family (publication)
EP 2821498 A1 20150107; EP 2821498 A4 20151209; EP 2821498 B1 20181212; JP 6176738 B2 20170809; JP WO2013128914 A1 20150730; US 2015111218 A1 20150423; US 9279809 B2 20160308; WO 2013128914 A1 20130906
DOCDB simple family (application)
EP 13755221 A 20130227; JP 2013001160 W 20130227; JP 2014502042 A 20130227; US 201314381116 A 20130227