EP 2831283 A4 20151104 - METHODS AND COMPOSITION FOR SEQUENCING MODIFIED NUCLEIC ACIDS
Title (en)
METHODS AND COMPOSITION FOR SEQUENCING MODIFIED NUCLEIC ACIDS
Title (de)
VERFAHREN UND ZUSAMMENSETZUNG ZUR SEQUENZIERUNG MODIFIZIERTER NUCLEINSÄUREN
Title (fr)
PROCÉDÉS ET COMPOSITION PERMETTANT LE SÉQUENÇAGE D'ACIDES NUCLÉIQUES MODIFIÉS
Publication
Application
Priority
- US 201261617999 P 20120330
- US 201261721206 P 20121101
- US 201261721339 P 20121101
- US 201361799237 P 20130315
- US 201361789354 P 20130315
- US 2013032816 W 20130318
Abstract (en)
[origin: WO2013148400A1] Methods, compositions, and systems are provided for characterization of modified nucleic acids. In certain preferred embodiments, single molecule sequencing methods are provided for identification of modified nucleotides within nucleic acid sequences. Modifications detectable by the methods provided herein include chemically modified bases, enzymatically modified bases, a basic sites, non-natural bases, secondary structures, and agents bound to a template nucleic acid.
IPC 8 full level
C12Q 1/68 (2006.01); G01N 33/48 (2006.01)
CPC (source: EP)
C12Q 1/6827 (2013.01); C12Q 1/6869 (2013.01); Y02A 90/10 (2017.12)
Citation (search report)
- [X] US 2011183320 A1 20110728 - FLUSBERG BENJAMIN [US], et al
- [A] WO 2010068289 A2 20100617 - PACIFIC BIOSCIENCES CALIFORNIA [US], et al
- [A] WO 2009145818 A1 20091203 - PACIFIC BIOSCIENCES CALIFORNIA [US], et al
- [A] WO 2008154317 A1 20081218 - PACIFIC BIOSCIENCES CALIFORNIA [US], et al
- [X] THOMPSON JOHN F ET AL: "The properties and applications of single-molecule DNA sequencing", GENOME BIOLOGY, BIOMED CENTRAL LTD., LONDON, GB, vol. 12, no. 2, 24 February 2011 (2011-02-24), pages 217, XP021091788, ISSN: 1465-6906
- [X] BENJAMIN FLUSBERG: "Direct detection of DNA methylation and mutagenic damage through single-molecule, real-time (SMRT (TM)) DNA sequencing", 101ST ANNUAL MEETING OF THE AMERICAN-ASSOCIATION-FOR-CANCER-RESEARCH; WASHINGTON, DC, USA; APRIL 17 -21, 2010, 1 April 2010 (2010-04-01), XP055215735, Retrieved from the Internet <URL:http://www.abstractsonline.com/Plan/ViewAbstract.aspx?mID=2521&sKey=ad61b071-5ab9-4f64-bf00-14f780960c07&cKey=68c67e8a-73b3-4b54-8a51-7bc1e5ae1748&mKey=0591fa3b-afef-49d2-8e65-55f41ee8117e> [retrieved on 20150923]
- [A] FLUSBERG BENJAMIN A ET AL: "Direct detection of DNA methylation during single-molecule, real-time sequencing", NATURE METHODS, NATURE PUBLISHING GROUP, GB, vol. 7, no. 6, 1 July 2010 (2010-07-01), pages 461 - 465, XP009142171, ISSN: 1548-7105, [retrieved on 20100509], DOI: 10.1038/NMETH.1459
- See references of WO 2013148400A1
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
DOCDB simple family (publication)
WO 2013148400 A1 20131003; AU 2013240166 A1 20141030; CA 2867489 A1 20131003; EP 2831283 A1 20150204; EP 2831283 A4 20151104
DOCDB simple family (application)
US 2013032816 W 20130318; AU 2013240166 A 20130318; CA 2867489 A 20130318; EP 13769322 A 20130318