EP 2834367 A4 20160323 - SAMPLE PREPARATION FOR FLOW CYTOMETRY
Title (en)
SAMPLE PREPARATION FOR FLOW CYTOMETRY
Title (de)
PROBENVORBEREITUNG FÜR DURCHFLUSSZYTOMETRIE
Title (fr)
PRÉPARATION D'ÉCHANTILLON POUR CYTOMÉTRIE DE FLUX
Publication
Application
Priority
- US 201261620823 P 20120405
- US 2013035291 W 20130404
Abstract (en)
[origin: WO2013152203A1] Described herein are methods and reagents for identifying and analyzing at least one microorganism (e.g. bacteria) in a sample and reducing the background signal intensity obtained when analyzing the sample by flow cytometry. The sample is prepared by combining the sample with a background signal-reducing molecule or with a nucleic acid stain covalently linked to a quencher. A portion of the particulate matter in the sample can optionally be removed with a resin prior to staining with a nucleic acid stain.
IPC 8 full level
C12Q 1/02 (2006.01); C12M 1/34 (2006.01); C12Q 1/68 (2006.01); G01N 33/48 (2006.01)
CPC (source: EP)
C12Q 1/06 (2013.01); C12Q 1/6806 (2013.01)
C-Set (source: EP)
Citation (search report)
- [X] US 5891738 A 19990406 - SOINI ERKKI [FI], et al
- [X] BARBESTI S ET AL: "TWO AND THREE-COLOR FLUOROESCENCE FLOW CYTOMETRIC ANALYSIS OF IMMUNOIDENTIFIED VIABLE BACTERIA", CYTOMETRY, ALAN LISS, NEW YORK, US, vol. 40, no. 3, 1 July 2000 (2000-07-01), pages 214 - 218, XP009042629, ISSN: 0196-4763, DOI: 10.1002/1097-0320(20000701)40:3<214::AID-CYTO6>3.0.CO;2-M
- [X] A. PIANETTI ET AL: "Determination of the Viability of Aeromonas hydrophila in Different Types of Water by Flow Cytometry, and Comparison with Classical Methods", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 71, no. 12, 1 December 2005 (2005-12-01), US, pages 7948 - 7954, XP055223542, ISSN: 0099-2240, DOI: 10.1128/AEM.71.12.7948-7954.2005
- [X] M. BERNEY: "Flow-cytometric study of vital cellular functions in Escherichia coli during solar disinfection (SODIS)", MICROBIOLOGY, vol. 152, no. 6, 1 June 2006 (2006-06-01), GB, pages 1719 - 1729, XP055223549, ISSN: 1350-0872, DOI: 10.1099/mic.0.28617-0
- [X] S. ALLEGRA ET AL: "Use of Flow Cytometry To Monitor Legionella Viability", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 74, no. 24, 10 October 2008 (2008-10-10), US, pages 7813 - 7816, XP055223546, ISSN: 0099-2240, DOI: 10.1128/AEM.01364-08
- [X] JUNG KYU CHOI ET AL: "3,3'-Diethylthiatricarbocyanine Iodide: A Highly Sensitive Chiroptical Reporter of DNA Helicity and Sequence", INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, vol. 12, no. 12, 16 November 2011 (2011-11-16), pages 8052 - 8062, XP055223668, DOI: 10.3390/ijms12118052
Citation (examination)
- US 5436134 A 19950725 - HAUGLAND RICHARD P [US], et al
- HUBERT ZIPPER ET AL: "Investigations on DNA intercalation and surface binding by SYBR Green I, its structure determination and methodological implications", NUCLEIC ACIDS RESEARCH, INFORMATION RETRIEVAL LTD, vol. 32, no. 12, 1 January 2004 (2004-01-01), pages e103 - 1, XP009140291, ISSN: 0305-1048
- MONIS P T ET AL: "Comparison of SYTO9 and SYBR Green I for real-time polymerase chain reaction and investigation of the effect of dye concentration on amplification and DNA melting curve analysis", ANALYTICAL BIOCHEMISTRY, ELSEVIER, AMSTERDAM, NL, vol. 340, no. 1, 1 May 2005 (2005-05-01), pages 24 - 34, XP022593917, ISSN: 0003-2697, [retrieved on 20050328], DOI: 10.1016/J.AB.2005.01.046
- See also references of WO 2013152203A1
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
DOCDB simple family (publication)
WO 2013152203 A1 20131010; AU 2013243378 A1 20141030; AU 2013243378 B2 20190411; CA 2869472 A1 20131010; CA 2869472 C 20170711; CN 104379760 A 20150225; CN 104379760 B 20180316; EP 2834367 A1 20150211; EP 2834367 A4 20160323; IN 8957DEN2014 A 20150522; JP 2015514403 A 20150521; JP 6401146 B2 20181003
DOCDB simple family (application)
US 2013035291 W 20130404; AU 2013243378 A 20130404; CA 2869472 A 20130404; CN 201380029083 A 20130404; EP 13773119 A 20130404; IN 8957DEN2014 A 20141027; JP 2015504727 A 20130404