EP 2844766 B1 20161123 - TARGETED DNA ENRICHMENT AND SEQUENCING
Title (en)
TARGETED DNA ENRICHMENT AND SEQUENCING
Title (de)
GEZIELTE DNA-ANREICHERUNG UND -SEQUENZIERUNG
Title (fr)
ENRICHISSEMENT ET SÉQUENÇAGE D'ADN CIBLÉ
Publication
Application
Priority
- EP 12166190 A 20120430
- EP 2013058934 W 20130429
- EP 13724179 A 20130429
Abstract (en)
[origin: WO2013164319A1] The invention relates to a method for enriching one or more target sequences of a deoxyribonucleic acid (DNA) in a composition, comprising the steps of providing a composition comprising one or more deoxyribonucleic acid (DNA) molecules, hybridizing to said one or more DNA molecules, one or more target specific ribonucleic acid (RNA) hybridization probes, thereby forming one or more RNA/DNA hybrids, capturing the RNA/DNA hybrids with one or more antibodies being specific for such RNA/DNA hybrids, thereby forming one or more RNA/DNA/antibody hybrids, isolating the one or more RNA/DNA/antibody hybrids, amplifying the one or more DNA molecules of the one or more RNA/DNA/antibody hybrids if necessary, and, optionally, sequencing the one or more DNA molecules of the one or more RNA/DNA/antibody hybrids or the amplification product, wherein the sequencing is preferably done by means of next generation sequencing. The invention also relates to a kit comprising a first an antibody which is specific for a DNA/RNA hybrid molecule, wherein optionally the antibody is bound to a magnetic particle, and additionally comprising one or more target specific RNA hybridization probes.
IPC 8 full level
C12Q 1/68 (2006.01)
CPC (source: CN EP US)
C12Q 1/6804 (2013.01 - CN EP US); C12Q 1/6806 (2013.01 - CN EP US); C12Q 1/6869 (2013.01 - US)
C-Set (source: CN EP US)
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
DOCDB simple family (publication)
WO 2013164319 A1 20131107; CN 104271770 A 20150107; EP 2844766 A1 20150311; EP 2844766 B1 20161123; JP 2015516814 A 20150618; US 2015141258 A1 20150521
DOCDB simple family (application)
EP 2013058934 W 20130429; CN 201380022651 A 20130429; EP 13724179 A 20130429; JP 2015509409 A 20130429; US 201314397733 A 20130429