EP 2859123 A2 20150415 - METHOD OF SEQUENCE DETERMINATION USING SEQUENCE TAGS
Title (en)
METHOD OF SEQUENCE DETERMINATION USING SEQUENCE TAGS
Title (de)
VERFAHREN ZUR SEQUENZBESTIMMUNG MITTELS SEQUENZTAGS
Title (fr)
PROCÉDÉ DE DÉTERMINATION DE SÉQUENCE À L'AIDE D'ÉTIQUETTES DE SÉQUENCE
Publication
Application
Priority
- US 201261658317 P 20120611
- US 201261738277 P 20121217
- US 201361776647 P 20130311
- US 201313835093 A 20130315
- US 201361829054 P 20130530
- US 2013045276 W 20130611
Abstract (en)
[origin: WO2013188471A2] The invention is directed to the use of sequence tags to improve sequence determination of amplicons of related sequences, particularly large and complex amplicons, such as those comprising recombined nucleic acids encoding immune receptor molecules. In one aspect, sequence reads having the same sequence tags are aligned after which final base calls are determined from a (possibly weighted) average base call from sequence read base calls at each position. Similarly, in another aspect, sequence reads comprising series of incorporation signals are aligned by common sequence tags and base calls in homopolymer regions are made as a function incorporation signal values at each "flow" position.
IPC 8 full level
C12Q 1/68 (2006.01)
CPC (source: CN EP)
C12Q 1/6869 (2013.01 - EP); C12Q 1/6874 (2013.01 - CN EP)
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
Designated extension state (EPC)
BA ME
DOCDB simple family (publication)
WO 2013188471 A2 20131219; WO 2013188471 A3 20140403; AU 2013274366 A1 20150122; CA 2875542 A1 20131219; CN 104520443 A 20150415; EP 2859123 A2 20150415; EP 2859123 A4 20151216; JP 2015519081 A 20150709; SG 11201407888R A 20141230
DOCDB simple family (application)
US 2013045276 W 20130611; AU 2013274366 A 20130611; CA 2875542 A 20130611; CN 201380042163 A 20130611; EP 13804085 A 20130611; JP 2015517372 A 20130611; SG 11201407888R A 20130611