Global Patent Index - EP 2859123 A4

EP 2859123 A4 20151216 - METHOD OF SEQUENCE DETERMINATION USING SEQUENCE TAGS

Title (en)

METHOD OF SEQUENCE DETERMINATION USING SEQUENCE TAGS

Title (de)

VERFAHREN ZUR SEQUENZBESTIMMUNG MITTELS SEQUENZTAGS

Title (fr)

PROCÉDÉ DE DÉTERMINATION DE SÉQUENCE À L'AIDE D'ÉTIQUETTES DE SÉQUENCE

Publication

EP 2859123 A4 20151216 (EN)

Application

EP 13804085 A 20130611

Priority

  • US 201261658317 P 20120611
  • US 201261738277 P 20121217
  • US 201361776647 P 20130311
  • US 201313835093 A 20130315
  • US 201361829054 P 20130530
  • US 2013045276 W 20130611

Abstract (en)

[origin: WO2013188471A2] The invention is directed to the use of sequence tags to improve sequence determination of amplicons of related sequences, particularly large and complex amplicons, such as those comprising recombined nucleic acids encoding immune receptor molecules. In one aspect, sequence reads having the same sequence tags are aligned after which final base calls are determined from a (possibly weighted) average base call from sequence read base calls at each position. Similarly, in another aspect, sequence reads comprising series of incorporation signals are aligned by common sequence tags and base calls in homopolymer regions are made as a function incorporation signal values at each "flow" position.

IPC 8 full level

C12Q 1/68 (2006.01)

CPC (source: CN EP)

C12Q 1/6869 (2013.01 - EP); C12Q 1/6874 (2013.01 - CN EP)

Citation (search report)

  • [XI] WO 2009017678 A2 20090205 - PACIFIC BIOSCIENCES CALIFORNIA [US], et al
  • [E] WO 2013130512 A2 20130906 - UNIV NORTH CAROLINA [US]
  • [A] WO 2012055929 A1 20120503 - ILLUMINA INC [US], et al
  • [AD] US 5846719 A 19981208 - BRENNER SYDNEY [GB], et al
  • [XI] C. B. JABARA ET AL: "Accurate sampling and deep sequencing of the HIV-1 protease gene using a Primer ID", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, vol. 108, no. 50, 30 November 2011 (2011-11-30), pages 20166 - 20171, XP055201609, ISSN: 0027-8424, DOI: 10.1073/pnas.1110064108
  • [A] MILLER JASON R ET AL: "Assembly algorithms for next-generation sequencing data", GENOMICS, ACADEMIC PRESS, SAN DIEGO, US, vol. 95, no. 6, 6 March 2010 (2010-03-06), pages 315 - 327, XP009165741, ISSN: 0888-7543
  • [A] MEGAN MANION ET AL: "Reducing Error in Next Generation Sequencing Data with NextGENe Software's Condensation Tool", March 2009 (2009-03-01), pages 1 - 3, XP055226038, Retrieved from the Internet <URL:http://www.softgenetics.com/PDF/ReducingError_NextGenerationSequencing_AppNote.pdf> [retrieved on 20151105]
  • See references of WO 2013188471A2

Designated contracting state (EPC)

AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

DOCDB simple family (publication)

WO 2013188471 A2 20131219; WO 2013188471 A3 20140403; AU 2013274366 A1 20150122; CA 2875542 A1 20131219; CN 104520443 A 20150415; EP 2859123 A2 20150415; EP 2859123 A4 20151216; JP 2015519081 A 20150709; SG 11201407888R A 20141230

DOCDB simple family (application)

US 2013045276 W 20130611; AU 2013274366 A 20130611; CA 2875542 A 20130611; CN 201380042163 A 20130611; EP 13804085 A 20130611; JP 2015517372 A 20130611; SG 11201407888R A 20130611