EP 2867250 A2 20150506 - PROINSULIN WITH ENHANCED HELPER SEQUENCE
Title (en)
PROINSULIN WITH ENHANCED HELPER SEQUENCE
Title (de)
PROINSULIN MIT HELFERSEQUENZ
Title (fr)
PROINSULINE COMPRENANT UNE SÉQUENCE AUXILIAIRE AMÉLIORÉE
Publication
Application
Priority
- EP 12002446 A 20120404
- EP 2013001003 W 20130404
- EP 13737127 A 20130404
Abstract (en)
[origin: WO2013149729A2] The invention provides a polypeptide comprising an insulin precursor and the N-terminal helper sequence MAKR. The helper sequence enhances the expression of the insulin precursor in a recombinant organism and increases the yields of insulin after purification and processing of the insulin precursor. The invention is thus further concerned with a method of producing insulin, in which proinsulin is expressed as recombinant polypeptide in an organism such as E. coli, isolated from the organism and then processed to yield active insulin.
IPC 8 full level
C07K 14/62 (2006.01)
CPC (source: EP)
C07K 14/62 (2013.01); C07K 2319/00 (2013.01); C07K 2319/21 (2013.01)
Citation (search report)
See references of WO 2013149729A2
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
Designated extension state (EPC)
BA ME
DOCDB simple family (publication)
WO 2013149729 A2 20131010; WO 2013149729 A3 20131205; EP 2867250 A2 20150506
DOCDB simple family (application)
EP 2013001003 W 20130404; EP 13737127 A 20130404