EP 2872652 A4 20160309 - DEVICES AND METHODS FOR NUCLEIC ACID PREPARATION AND ANALYSIS
Title (en)
DEVICES AND METHODS FOR NUCLEIC ACID PREPARATION AND ANALYSIS
Title (de)
VORRICHTUNGEN UND VERFAHREN ZUR NUKLEINSÄUREHERSTELLUNG UND -ANALYSE
Title (fr)
DISPOSITIFS ET MÉTHODES DE PRÉPARATION ET D'ANALYSE D'ACIDES NUCLÉIQUES
Publication
Application
Priority
- US 201261625743 P 20120418
- US 201261635221 P 20120418
- US 201261625745 P 20120418
- US 201261635263 P 20120418
- US 201361784399 P 20130314
- US 201361783601 P 20130314
- US 201361784061 P 20130314
- US 201361790721 P 20130315
- US 2013037140 W 20130418
Abstract (en)
[origin: WO2013158860A1] The invention provides in part a device for preparing a fluid sample, including but not limited to a sample comprising genomic DNA. The invention also provides in part structures and methods that allow polymers of any length, including nucleic acids, to be stretched into a long, linear conformation for further analysis. The invention also provides in part devices and methods for improving labeling of nucleic acids including intercalation of nucleic acids using for example mono intercalators such as mono cyanine intercalators. The invention also provides in part methods for analyzing populations nucleic acids including nucleic acids from different subjects or different samples such as bacteria in biomes, and for identifying nucleic acids present in such populations. A non-limiting example is an analysis of bacteria in biomes. Another example is an analysis of DNA from different human subjects.
IPC 8 full level
C12Q 1/68 (2006.01); C12N 15/10 (2006.01); C12N 15/11 (2006.01); G01N 35/08 (2006.01)
CPC (source: EP)
C12Q 1/6816 (2013.01); B01L 2200/0636 (2013.01); B01L 2200/0663 (2013.01); B01L 2300/0816 (2013.01); B01L 2300/0867 (2013.01); B01L 2400/0415 (2013.01); B01L 2400/049 (2013.01)
Citation (search report)
- [I] WO 0009757 A1 20000224 - U S GENOMICS [US], et al
- [I] WO 0113088 A1 20010222 - US GENOMICS INC [US]
- [I] WO 2004091795 A2 20041028 - US GENOMICS INC [US], et al
- [I] CHAN E Y ET AL: "DNA mapping using microfluidic stretching and single-molecule detection of fluorescent site-specific tags", GENOME RESEARCH, COLD SPRING HARBOR LABORATORY PRESS, vol. 14, no. 6, 1 June 2004 (2004-06-01), pages 1137 - 1146, XP002346048, ISSN: 1088-9051, DOI: 10.1101/GR.1635204
- [A] BAKAJIN O B ET AL: "ELECTROHYDRODYNAMIC STRETCHING OF DNA IN CONFINED ENVIRONMENTS", PHYSICAL REVIEW LETTERS, AMERICAN PHYSICAL SOCIETY, US, vol. 80, no. 12, 23 March 1998 (1998-03-23), pages 2737 - 2740, XP000980688, ISSN: 0031-9007, DOI: 10.1103/PHYSREVLETT.80.2737
- See references of WO 2013158860A1
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
DOCDB simple family (publication)
WO 2013158860 A1 20131024; EP 2872652 A1 20150520; EP 2872652 A4 20160309
DOCDB simple family (application)
US 2013037140 W 20130418; EP 13778675 A 20130418