EP 2880016 A1 20150610 - FUNCTIONALISATION METHODS AND REAGENTS USED IN SUCH METHODS USING AN AZAISATOIC ANHYDRIDE OR ONE OF THE DERIVATIVES OF SAME, BIOLOGICAL MOLECULES THEREBY TREATED, AND KITS
Title (en)
FUNCTIONALISATION METHODS AND REAGENTS USED IN SUCH METHODS USING AN AZAISATOIC ANHYDRIDE OR ONE OF THE DERIVATIVES OF SAME, BIOLOGICAL MOLECULES THEREBY TREATED, AND KITS
Title (de)
FUNKTIONALISIERUNGSVERFAHREN UND REAGENZIEN FÜR DIESE VERFAHREN UNTER VERWENDUNG EINES AZAISATOISCHEN ANHYDRIDS ODER EINES DERIVATES DAVON, DAMIT BEHANDELTE BIOLOGISCHE MOLEKÜLE UND KITS
Title (fr)
PROCEDES DE FONCTIONNALISATION ET REACTIFS UTILISES DANS DE TELS PROCEDES UTILISANT UN ANHYDRIDE AZA-ISATOÏQUE OU UN DE SES DERIVES, MOLECULES BIOLOGIQUES AINSI TRAITEES ET KITS
Publication
Application
Priority
- FR 1257526 A 20120802
- EP 2013065854 W 20130726
Abstract (en)
[origin: WO2014019966A1] The present invention concerns a method for functionalising at least one ribonucleic acid (RNA) molecule, contained in a liquid sample, which comprises the following steps: a) providing at least: - one binding molecule constituted by an azaisatoic anhydride or one of the derivatives of same, - a moiety of interest, and - a binding arm connecting the binding molecule with the moiety of interest, b) causing the anhydride function of the binding molecule to react with at least one hydroxyl moiety carried: - in position 2' of the ribose of one of the RNA nucleotides, and/or - in position(s) 2' and/or 3' of the ribose of the nucleotide at terminal end 3' of the RNA, and c) obtaining an aza-anthranylate that binds the RNA to the moiety of interest via the binding arm.
IPC 8 full level
C07D 213/80 (2006.01); C07D 495/04 (2006.01); C07D 519/00 (2006.01); C07H 21/00 (2006.01); C12Q 1/68 (2006.01)
CPC (source: EP US)
C07D 213/80 (2013.01 - EP US); C07D 405/02 (2013.01 - EP US); C07D 495/04 (2013.01 - EP US); C07D 519/00 (2013.01 - EP US); C07H 1/00 (2013.01 - EP US); C07H 21/02 (2013.01 - EP US); C12Q 1/6825 (2013.01 - US)
Citation (search report)
See references of WO 2014019966A1
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
Designated extension state (EPC)
BA ME
DOCDB simple family (publication)
WO 2014019966 A1 20140206; EP 2880016 A1 20150610; FR 2994184 A1 20140207; FR 2994184 B1 20171208; JP 2015526076 A 20150910; JP 6407151 B2 20181017; US 10174068 B2 20190108; US 2015210732 A1 20150730; US 2019112329 A1 20190418
DOCDB simple family (application)
EP 2013065854 W 20130726; EP 13744493 A 20130726; FR 1257526 A 20120802; JP 2015524746 A 20130726; US 201314416996 A 20130726; US 201816217483 A 20181212