EP 2895592 A1 20150722 - A YEAST RECOMBINANT CELL CAPABLE OF PRODUCING GDP-FUCOSE
Title (en)
A YEAST RECOMBINANT CELL CAPABLE OF PRODUCING GDP-FUCOSE
Title (de)
REKOMBINANTE HEFEZELLE ZUR PRODUKTION VON GDP-FUCOSE
Title (fr)
CELLULE DE LEVURE RECOMBINÉE CAPABLE DE PRODUIRE DU GDP-FUCOSE
Publication
Application
Priority
- US 201161466639 P 20110323
- EP 2012055245 W 20120323
Abstract (en)
[origin: WO2012127045A1] A yeast strain expressing a bi-functional fucokinase/GDP-L-fucose pyrophosphorylase enzyme and capable of producing GDP-L-fucose in vivo is provided. Also provided are yeast cells which express a GDP-L-fucose transporter and /or a fucosyl transferase with the bi-functional enzyme. In addition, the said yeast contains one or more expression cassettes for fusion proteins of heterologous glycosylation pathway and an ER/Golgi retention sequence. Finally, the invention also provides a method for producing recombinant target glycoproteins.
IPC 8 full level
C12N 1/16 (2006.01); C12N 9/10 (2006.01); C12N 9/12 (2006.01)
CPC (source: EP)
C12N 9/1051 (2013.01); C12N 9/1241 (2013.01); C12P 21/005 (2013.01); C12Y 207/0703 (2013.01)
Citation (search report)
See references of WO 2012127045A1
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
Designated extension state (EPC)
BA ME
DOCDB simple family (publication)
WO 2012127045 A1 20120927; EP 2895592 A1 20150722; JP 2014509864 A 20140424
DOCDB simple family (application)
EP 2012055245 W 20120323; EP 12710943 A 20120323; JP 2014500415 A 20120323