EP 2906518 A4 20160727 - IN VIVO AND IN VITRO OLEFIN CYCLOPROPANATION CATALYZED BY HEME ENZYMES
Title (en)
IN VIVO AND IN VITRO OLEFIN CYCLOPROPANATION CATALYZED BY HEME ENZYMES
Title (de)
DURCH HÄMENZYME KATALYSIERTE IN-VIVO- UND IN-VITRO-OLEFINCYCLOPROPANIERUNG
Title (fr)
CYCLOPROPANATION D'OLÉFINE IN VIVO ET IN VITRO CATALYSÉE PAR DES ENZYMES HÈMES
Publication
Application
Priority
- US 201261711640 P 20121009
- US 201261740247 P 20121220
- US 201361784917 P 20130314
- US 201361815997 P 20130425
- US 201361818329 P 20130501
- US 201361838167 P 20130621
- US 201361856493 P 20130719
- US 2013063577 W 20131004
Abstract (en)
[origin: WO2014058744A2] The present invention provides methods for catalyzing the conversion of an olefin to any compound containing one or more cyclopropane functional groups using heme enzymes. In certain aspects, the present invention provides a method for producing a cyclopropanation product comprising providing an olefinic substrate, a diazo reagent, and a heme enzyme; and admixing the components in a reaction for a time sufficient to produce a cyclopropanation product. In other aspects, the present invention provides heme enzymes including variants and fragments thereof that are capable of carrying out in vivo and in vitro olefin cyclopropanation reactions. Expression vectors and host cells expressing the heme enzymes are also provided by the present invention.
IPC 8 full level
C07C 2/52 (2006.01); C12N 9/02 (2006.01); C12P 7/62 (2006.01)
CPC (source: EP US)
C12N 9/0004 (2013.01 - EP US); C12N 9/0042 (2013.01 - EP US); C12P 7/62 (2013.01 - EP US); C12P 13/02 (2013.01 - US); C12Y 106/02004 (2013.01 - EP US); Y02P 20/52 (2015.11 - US)
Citation (search report)
- [X] NICOLAS ET AL: "Asymmetric catalytic cyclopropanation reactions in water", COORDINATION CHEMISTRY REVIEWS, ELSEVIER SCIENCE, AMSTERDAM, NL, vol. 252, no. 5-7, 27 February 2008 (2008-02-27), pages 727 - 735, XP022500964, ISSN: 0010-8545, DOI: 10.1016/J.CCR.2007.09.003
- [XP] P. S. COELHO ET AL: "Olefin Cyclopropanation via Carbene Transfer Catalyzed by Engineered Cytochrome P450 Enzymes", SCIENCE, vol. 339, no. 6117, 18 January 2013 (2013-01-18), US, pages 307 - 310, XP055252970, ISSN: 0036-8075, DOI: 10.1126/science.1231434
- See references of WO 2014058744A2
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
DOCDB simple family (publication)
WO 2014058744 A2 20140417; WO 2014058744 A3 20140619; EP 2906518 A2 20150819; EP 2906518 A4 20160727; JP 2015534464 A 20151203; US 10208322 B2 20190219; US 11008596 B2 20210518; US 2014242647 A1 20140828; US 2016002682 A1 20160107; US 2017247725 A1 20170831; US 2019211367 A1 20190711; US 8993262 B2 20150331; US 9493799 B2 20161115
DOCDB simple family (application)
US 2013063577 W 20131004; EP 13845063 A 20131004; JP 2015536817 A 20131004; US 201414185861 A 20140220; US 201514625449 A 20150218; US 201615278561 A 20160928; US 201916246246 A 20190111