EP 2906707 B1 20190724 - METHOD OF MAKING POLYHYDROXYALKANOATE COPOLYMERS (3HB-CO-4HB)
Title (en)
METHOD OF MAKING POLYHYDROXYALKANOATE COPOLYMERS (3HB-CO-4HB)
Title (de)
VERFAHREN ZUR HERSTELLUNG VON POLYHYDROXYALKANOAT-COPOLYMEREN (3HB-CO-4HB)
Title (fr)
PROCÉDÉ DE PRODUCTION DE COPOLYMÈRES DE POLYHYDROXYALCANOATE (3HB-CO-4HB)
Publication
Application
Priority
- US 201261711825 P 20121010
- US 201261734489 P 20121207
- US 2013062812 W 20131001
Abstract (en)
[origin: WO2014058655A1] A polyhydroxyalkanoate copolymer composition is provided. The composition comprises a plurality of polyhydroxyalkanoate copolymer molecules. The polyhydroxyalkanoate copolymer molecules (i) comprise 3-hydroxybutyrate monomers and 4-hydroxybutyrate monomers, (ii) have a monomeric molar percentage of 4-hydroxybutyrate monomers of 23.5 to 75%, and (iii) have a biobased content of ≥ 80%. Also provided is a method of making a polyhydroxyalkanoate copolymer composition. The method comprises culturing an organism in the presence of one or more carbon raw materials under conditions under which (a) the one or more carbon raw materials are converted to 3- hydroxybutyryl-CoA and 44iydroxybutyryl-CoA and (b) the 34iydroxybutyryl-CoA and the 4- hydroxybutyryl-CoA are polymerized to form the polyhydroxyalkanoate copolymer molecules, thereby forming the composition. The organism has been genetically engineered to comprise particular enzymatic activities, and to not comprise other particular enzymatic activities. The one or more carbon raw materials, taken together, have a biobased content of ≥80%.
IPC 8 full level
C08L 67/04 (2006.01); C12P 7/62 (2006.01)
CPC (source: CN EP US)
C08G 63/06 (2013.01 - CN EP US); C08L 67/04 (2013.01 - US); C12P 7/625 (2013.01 - CN EP US); C08L 2201/06 (2013.01 - US)
Citation (examination)
- WO 2013184822 A1 20131212 - METABOLIX INC [US]
- WO 2013184836 A1 20131212 - METABOLIX INC [US]
- MARTINEZ-MORALES ET AL: "Chromosomal integration of heterologous DNA in Escherichia coli with precise removal of markers and replicons used during construction", JOURNAL OF BACTERIOLOGY, vol. 181, 1999, pages 7143 - 7148, XP002156609
- HASUNUMA ET AL: "Effect of dna mutations on the replication of plasmid pSC101 in Escherichia coli K-12", JOURNAL OF BACTERIOLOGY, vol. 137, 1979, pages 1095 - 1099, XP008013894
- SMITH ET AL: "Bacterial fitness and plasmid loss: the importance of culture conditions and plasmid size", CANADIAN JOURNAL OF MICROBIOLOGY, vol. 44, 1998, pages 351 - 355
- LENSKI ET AL: "Effects of segregation and selection on instability of plasmid pACYC184 in Escherichia coli B", JOURNAL OF BACTERIOLOGY, vol. 169, 1987, pages 5314 - 5316
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
DOCDB simple family (publication)
WO 2014058655 A1 20140417; WO 2014058655 A8 20150514; BR 112015007560 A2 20170808; BR 112015007560 A8 20171003; BR 112015007560 B1 20210831; CN 104718297 A 20150617; CN 104718297 B 20170929; EP 2906707 A1 20150819; EP 2906707 B1 20190724; ES 2747230 T3 20200310; US 10323261 B2 20190618; US 2015240273 A1 20150827; US 2018057848 A1 20180301
DOCDB simple family (application)
US 2013062812 W 20131001; BR 112015007560 A 20131001; CN 201380052531 A 20131001; EP 13777398 A 20131001; ES 13777398 T 20131001; US 201314434651 A 20131001; US 201715785845 A 20171017