EP 2909318 A1 20150826 - A THERMOSTABLE SUCROSE AND SUCROSE-6'-PHOSPHATE PHOSPHORYLASE
Title (en)
A THERMOSTABLE SUCROSE AND SUCROSE-6'-PHOSPHATE PHOSPHORYLASE
Title (de)
WÄRMESTABILE SACCHAROSE UND SACCHAROSE-6 '-PHOSPHAT-PHOSPHORYLASE
Title (fr)
SACCHAROSE ET PHOSPHORYLASE DE SACCHAROSE-6'-PHOSPHATE THERMOSTABLES
Publication
Application
Priority
- EP 12188966 A 20121018
- EP 13159683 A 20130318
- EP 2013071587 W 20131016
- EP 13776826 A 20131016
Abstract (en)
[origin: WO2014060452A1] The present invention relates to the identification, recombinant expression and characterization of a sucrose and sucrose-6'-phosphate phosphorylase of the thermophilic bacterium Thermoanaerobacterium thermosaccharolyticum. The enzyme has an optimal temperature of about 55°C and has a high specificity for its substrates. More importantly, the sucrose and sucrose-6'-phosphate phosphorylase of the present invention has a half-life of at least 60 hours at the industrially relevant temperature of about 60°C. This enzyme is thus -for example-useful for the industrial production of D-fructose,D-fructose-6-phosphate,alpha-D-glucose -1-phosphate or alpha-D-glucosides.
IPC 8 full level
C07K 14/00 (2006.01); C12N 5/00 (2006.01); C12N 9/10 (2006.01)
CPC (source: EP)
C12N 9/1051 (2013.01); C12N 9/1066 (2013.01); C12P 19/44 (2013.01); C12Y 204/01007 (2013.01); C12Y 204/01014 (2013.01)
Citation (search report)
See references of WO 2014060452A1
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
Designated extension state (EPC)
BA ME
DOCDB simple family (publication)
DOCDB simple family (application)
EP 2013071587 W 20131016; EP 13776826 A 20131016