EP 2914960 B1 20170712 - DETECTING CELLS SECRETING A PROTEIN OF INTEREST
Title (en)
DETECTING CELLS SECRETING A PROTEIN OF INTEREST
Title (de)
NACHWEIS VON EIN PROTEIN VON INTERESSE AUSSCHEIDENDEN ZELLEN
Title (fr)
DÉTECTION DE CELLULES SÉCRÉTANT UNE PROTÉINE D'INTÉRÊT
Publication
Application
Priority
- US 201261720349 P 20121030
- US 201314044559 A 20131002
- US 2013067324 W 20131029
Abstract (en)
[origin: US2014120558A1] In some cases, the described systems and methods include obtaining a cell sample containing multiple antibody-producing cells. In such cases, the cells can be tagged with a cross-linking reagent having a first portion configured to bind to a marker on the antibody-producing cells and a second portion configured to bind to an antigen of interest. In some instances, the tagged antibody-producing cells are exposed to the antigen of interest such that the antigen becomes bound to the cells. In some such instances, the antibody-producing cells are also allowed to produce an antibody, such that a portion of the antibody-producing cells produce an antigen-specific antibody that binds to the antigen of interest. To identify cells that produce the antigen-specific antibody, the tagged cells can be exposed to a labeled secondary antibody that is configured to bind to the antigen-specific antibody. Other implementations are also described.
IPC 8 full level
G01N 33/53 (2006.01); C12Q 1/04 (2006.01)
CPC (source: EP US)
G01N 33/56911 (2013.01 - US); G01N 33/56961 (2013.01 - US); G01N 33/56966 (2013.01 - US); G01N 33/56972 (2013.01 - EP US); G01N 33/6854 (2013.01 - EP US); G01N 2458/00 (2013.01 - US); G01N 2469/10 (2013.01 - US)
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
DOCDB simple family (publication)
US 2014120558 A1 20140501; US 8921055 B2 20141230; EP 2914960 A1 20150909; EP 2914960 A4 20160518; EP 2914960 B1 20170712; JP 2015535344 A 20151210; JP 6345681 B2 20180620; US 2015111784 A1 20150423; US 9588117 B2 20170307; WO 2014070783 A1 20140508
DOCDB simple family (application)
US 201314044559 A 20131002; EP 13850928 A 20131029; JP 2015539921 A 20131029; US 2013067324 W 20131029; US 201414583351 A 20141226