Global Patent Index - EP 2932270 B1

EP 2932270 B1 20181024 - PHOTOACTIVATED CHEMICAL BLEACHING OF DYES

Title (en)

PHOTOACTIVATED CHEMICAL BLEACHING OF DYES

Title (de)

LICHTAKTIVIERTE CHEMISCHE BLEICHUNG VON FARBSTOFFEN

Title (fr)

BLANCHIMENT CHIMIQUE PHOTOACTIVÉ DE COLORANTS

Publication

EP 2932270 B1 20181024 (EN)

Application

EP 13814736 A 20131211

Priority

  • US 201261735586 P 20121211
  • US 2013074328 W 20131211

Abstract (en)

[origin: WO2014093455A1] Methods comprising the use of photoactivated chemical bleaching for detecting multiple targets in a biological sample are provided. The methods include the steps of providing a biological sample including multiple targets, binding at least one probe to one or more target present in the sample, and detecting a signal from the probe. The method further includes the steps of contacting the sample comprising the bound probe with an electron transfer reagent, as well as an optional additive which prevents target modification during photoactivated chemical bleaching, and irradiating the sample, thereby initiating a photoreaction that substantially inactivates the probe by photoactivated chemical bleaching. The method further includes the steps of binding at least one probe to one or more target present in the sample, and detecting a signal from the probe. The process of binding, detecting and bleaching may be iteratively repeated.

IPC 8 full level

G01N 21/75 (2006.01); G01N 33/53 (2006.01); G01N 33/58 (2006.01)

CPC (source: EP US)

G01N 21/75 (2013.01 - US); G01N 33/5306 (2013.01 - US); G01N 33/582 (2013.01 - EP US)

Designated contracting state (EPC)

AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

DOCDB simple family (publication)

WO 2014093455 A1 20140619; AU 2013359393 A1 20150604; AU 2013359393 B2 20190103; BR 112015013403 A2 20170711; CA 2893953 A1 20140619; CN 105143885 A 20151209; CN 105143885 B 20190712; EP 2932270 A1 20151021; EP 2932270 B1 20181024; JP 2016508213 A 20160317; JP 6335187 B2 20180530; KR 20150095755 A 20150821; NZ 708935 A 20190531; RU 2015120176 A 20170119; SG 11201504129S A 20150629; US 10175174 B2 20190108; US 2015316482 A1 20151105

DOCDB simple family (application)

US 2013074328 W 20131211; AU 2013359393 A 20131211; BR 112015013403 A 20131211; CA 2893953 A 20131211; CN 201380072726 A 20131211; EP 13814736 A 20131211; JP 2015545948 A 20131211; KR 20157018103 A 20131211; NZ 70893513 A 20131211; RU 2015120176 A 20131211; SG 11201504129S A 20131211; US 201314648176 A 20131211