EP 2964757 A1 20160113 - IMPROVEMENT OF CLOSTRIDIAL BUTANOL PRODUCTION BY GENE OVEREXPRESSION
Title (en)
IMPROVEMENT OF CLOSTRIDIAL BUTANOL PRODUCTION BY GENE OVEREXPRESSION
Title (de)
VERBESSERUNG DER HERSTELLUNG VON CLOSTRIDIELLEM BUTANOL DURCH GENÜBEREXPRESSION
Title (fr)
AMÉLIORATION DE LA PRODUCTION DE BUTANOL CLOSTRIDIAL PAR SUREXPRESSION GÉNIQUE
Publication
Application
Priority
- EP 13158012 A 20130306
- EP 2014054334 W 20140306
- EP 14708052 A 20140306
Abstract (en)
[origin: EP2774986A1] The present invention relates to metabolic engineering of Clostridium acetobutylicum. A Clostridium cell with the ability to produce butanol, wherein the cell comprises a genetic modification that results in overexpression of one or more of the following genes: — a gene encoding crotonase ( crt ), — a gene encoding butyryl-CoA dehydrogenase ( bcd ), — a gene encoding 3-hydroxybutyryl-CoA dehydrogenase ( hbd ). Overexpression of any of the two enzymes increased the speed of butanol production for at least factor of two, resulting in increase of final butanol titer for at least 28%.
IPC 8 full level
C12N 9/04 (2006.01); C12N 9/88 (2006.01); C12P 7/16 (2006.01)
CPC (source: EP)
C12N 9/0006 (2013.01); C12N 9/88 (2013.01); C12N 15/52 (2013.01); C12P 7/16 (2013.01); Y02E 50/10 (2013.01)
Citation (search report)
See references of WO 2014135633A1
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
Designated extension state (EPC)
BA ME
DOCDB simple family (publication)
EP 2774986 A1 20140910; EP 2964757 A1 20160113; WO 2014135633 A1 20140912
DOCDB simple family (application)
EP 13158012 A 20130306; EP 14708052 A 20140306; EP 2014054334 W 20140306