EP 2971086 A1 20160120 - FUNCTIONALIZED 3-ALKYNYL PYRAZOLOPYRIMIDINE ANALOGUES AS UNIVERSAL BASES AND METHODS OF USE
Title (en)
FUNCTIONALIZED 3-ALKYNYL PYRAZOLOPYRIMIDINE ANALOGUES AS UNIVERSAL BASES AND METHODS OF USE
Title (de)
FUNKTIONALISIERTE 3-ALKYNYL-PYRAZOLOPYRIMIDIN-ANALOGA ALS UNIVERSELLE BASEN UND VERFAHREN ZUR VERWENDUNG
Title (fr)
ANALOGUES DE 3-ALCYNYL PYRAZOLOPYRIMIDINES FONCTIONNALISÉS UTILISÉS EN TANT QUE BASES UNIVERSELLES ET PROCÉDÉS D'UTILISATION
Publication
Application
Priority
- US 201313827456 A 20130314
- US 2014021799 W 20140307
Abstract (en)
[origin: WO2014159063A1] 3-alkynyl inosine analogs and their uses as universal bases are provided. The inosine analogues can be incorporated into nucleic acid primers and probes. They do not significantly destabilize nucleic acid duplexes. As a result, the novel nucleic acid primers and probes incorporating the inosine analogues can be used in a variety of methods. The analogs function unexpectedly well as universal bases. Not only do they stabilize duplexes substantially more than hypoxanthine opposite A, C, T, and G but they are also recognized in primers by polymerases, allowing efficient amplification.
IPC 8 full level
C12Q 1/68 (2006.01)
CPC (source: EP)
C07H 21/00 (2013.01); C12Q 1/6806 (2013.01); C12Q 1/6846 (2013.01); C12Q 2525/101 (2013.01)
Citation (search report)
See references of WO 2014159063A1
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
Designated extension state (EPC)
BA ME
DOCDB simple family (publication)
DOCDB simple family (application)
US 2014021799 W 20140307; EP 14714494 A 20140307