EP 3013979 A1 20160504 - DIRECT, PROGRAMMABLE DETECTION OF EPIGENETIC DNA CYTOSINE MODIFICATIONS USING TAL EFFECTORS
Title (en)
DIRECT, PROGRAMMABLE DETECTION OF EPIGENETIC DNA CYTOSINE MODIFICATIONS USING TAL EFFECTORS
Title (de)
DIREKTE ERKENNUNG VON EPIGENETISCHER DNA-CYTOSINMODIFIKATION UNTER VERWENDUNG VON TAL-EFFEKTOREN UND DNA-POLYMERASEREAKTIONEN
Title (fr)
DÉTECTION DIRECTE DE MODIFICATION DE CYTOSINE D'ADN ÉPIGÉNÉTIQUE UTILISANT DES EFFECTEURS TAL ET DES RÉACTIONS DE POLYMÉRASE D'ADN
Publication
Application
Priority
- EP 13003252 A 20130626
- EP 2014001753 W 20140626
- EP 14747293 A 20140626
Abstract (en)
[origin: WO2014206568A1] The present invention relates to methods for the differentiation of a 5-hydroxymethyl modification of a cytosine residue of interest in a nucleic acid molecule from (i) a 5-methyl modification of said cytosine residue of interest o,r (ii) said unmodified cytosine residue of interest, said methods making use of the capability of transcription-activator-like effector (TALE) proteins to preferentially bind with strong affinity to nucleic acid sequences containing non-modified cytosine residues or 5-methyl modified cytosine residues, and to bind, if at all, with only strongly reduced affinity to nucleic acid sequences containing 5-hydroxymethyl modified cytosine residues. The present invention further relates to respective uses of TALE proteins for the differentiation of a 5-hydroxymethyl modification of a cytosine residue of interest in a nucleic acid molecule from (i) a 5-methyl modification of said cytosine residue of interest or (ii) said unmodified cytosine residue of interest.
IPC 8 full level
C12Q 1/68 (2006.01)
CPC (source: EP US)
C12Q 1/6827 (2013.01 - EP US); C12Q 1/6858 (2013.01 - EP US)
Citation (search report)
See references of WO 2014206568A1
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
Designated extension state (EPC)
BA ME
DOCDB simple family (publication)
WO 2014206568 A1 20141231; EP 3013979 A1 20160504; EP 3013979 B1 20170405; US 2016138094 A1 20160519; US 9879315 B2 20180130
DOCDB simple family (application)
EP 2014001753 W 20140626; EP 14747293 A 20140626; US 201414899483 A 20140626