EP 3019904 A1 20160518 - ASSEMBLY FOR LIGHT SHEET MICROSCOPY
Title (en)
ASSEMBLY FOR LIGHT SHEET MICROSCOPY
Title (de)
ANORDNUNG ZUR LICHTBLATTMIKROSKOPIE
Title (fr)
SYSTÈME DE MICROSCOPIE À FEUILLE DE LUMIÈRE
Publication
Application
Priority
- DE 102013107297 A 20130710
- EP 2014064551 W 20140708
Abstract (en)
[origin: WO2015004108A1] The invention relates to an assembly for light sheet microscopy. Said assembly comprises a sample container (1) for receiving a sample (3) present in a medium (2). The sample container (1) is aligned in respect of a flat reference surface. The assembly further comprises illumination optics having an illumination lens (6) for illuminating the sample (3) by means of a light sheet, wherein the optical axis (7) of the illuminating lens (6) and the light sheet lie in a plane which, together with the normals of the reference surface, includes an illumination angle (ß) different from zero, and detection optics having a detection lens (8), the optical axis (9) thereof together with the normals of the reference surface including a detection angle (δ) different from zero. According to the invention said assembly has a separating layer system of one or a plurality of layers of specified thicknesses and made of specified materials for spatial separation of the medium (2), in which the sample (3) is present, from the illumination lens (6) and the detection lens (8), wherein the separating layer system, together with a boundary surface (11) aligned parallel to the reference surface, at least in the region accessible for the illumination lens (6) and the detection lens (8) for the illumination and the detection, contacts the medium (2). According to the invention, the illumination angle (ß) and detection angle (δ) are specified based on numeric apertures (NAD, NAB) of the detection lens (8) or the illumination lens (6).
IPC 8 full level
G02B 21/06 (2006.01); G01N 21/03 (2006.01); G02B 21/16 (2006.01); G02B 21/34 (2006.01); G02B 21/36 (2006.01)
CPC (source: EP US)
G01N 21/15 (2013.01 - US); G02B 21/0032 (2013.01 - EP US); G02B 21/0076 (2013.01 - EP US); G02B 21/0088 (2013.01 - US); G02B 21/02 (2013.01 - US); G02B 21/16 (2013.01 - EP US); G02B 21/33 (2013.01 - US); G02B 21/34 (2013.01 - EP US); G02B 21/367 (2013.01 - EP US); G02B 27/0025 (2013.01 - US); G02B 27/0068 (2013.01 - US); G01N 2021/0396 (2013.01 - EP US)
Citation (search report)
See references of WO 2015004108A1
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
Designated extension state (EPC)
BA ME
DOCDB simple family (publication)
DE 102013107297 A1 20150115; CN 105359025 A 20160224; CN 105359025 B 20190125; EP 3019904 A1 20160518; JP 2016525229 A 20160822; JP 6492073 B2 20190327; US 10712553 B2 20200714; US 2016154236 A1 20160602; WO 2015004108 A1 20150115
DOCDB simple family (application)
DE 102013107297 A 20130710; CN 201480038895 A 20140708; EP 14735991 A 20140708; EP 2014064551 W 20140708; JP 2016524791 A 20140708; US 201414904067 A 20140708