EP 3025151 B1 20220216 - METHOD AND SYSTEM OF MICROFLUIDIC IMMUNOASSAY USING MAGNETIC BEADS
Title (en)
METHOD AND SYSTEM OF MICROFLUIDIC IMMUNOASSAY USING MAGNETIC BEADS
Title (de)
VERFAHREN UND SYSTEM FÜR EIN MIKROFLUIDISCHES IMMUNOASSAY UNTER VERWENDUNG MAGNETISCHER KÜGELCHEN
Title (fr)
PROCÉDÉ ET SYSTÈME D'IMMUNODOSAGE MICROFLUIDIQUE AU MOYEN DE BILLES MAGNÉTIQUES
Publication
Application
Priority
- US 201461927960 P 20140115
- US 2015011659 W 20150115
Abstract (en)
[origin: US2015198593A1] A microfluidic Western blot method and system including a microfluidic western blot method for immunoassay of proteins, the method including introducing a sample including the proteins onto a chip; electrophoretically separating the proteins; binding the separated proteins to beads to form protein-attached beads, the beads being magnetic; flowing the protein-attached beads into a magnetic holding region; applying a magnetic field to the magnetic holding region to fix the protein-attached beads in place within the magnetic holding region; binding primary antibodies to target proteins on the protein-attached beads; binding secondary antibodies to the bound primary antibodies; and detecting the bound secondary antibodies.
IPC 8 full level
G01N 27/447 (2006.01); G01N 33/53 (2006.01)
CPC (source: EP US)
G01N 27/447 (2013.01 - EP); G01N 33/5302 (2013.01 - EP US); G01N 33/54326 (2013.01 - EP US); G01N 33/561 (2013.01 - EP US)
Citation (examination)
- WO 2010041230 A2 20100415 - CNRS DAE [FR], et al
- US 5736410 A 19980407 - ZARLING DAVID A [US], et al
- US 2009018029 A1 20090115 - MIAO ZHENWEI [US], et al
- US 2006292647 A1 20061228 - GREEN LAWRENCE R [US], et al
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
DOCDB simple family (publication)
US 2015198593 A1 20150716; US 9835623 B2 20171205; CN 105992948 A 20161005; CN 113533485 A 20211022; EP 3025151 A1 20160601; EP 3025151 B1 20220216; EP 4086618 A1 20221109; US 10877032 B2 20201229; US 2018149647 A1 20180531; US 2021080457 A1 20210318; WO 2015109126 A1 20150723
DOCDB simple family (application)
US 201514597999 A 20150115; CN 201580004767 A 20150115; CN 202110788786 A 20150115; EP 15704409 A 20150115; EP 22156907 A 20150115; US 2015011659 W 20150115; US 201715830777 A 20171204; US 202017104967 A 20201125