EP 3033647 A1 20160622 - HIGH-RESOLUTION 3D FLUORESCENCE MICROSCOPY
Title (en)
HIGH-RESOLUTION 3D FLUORESCENCE MICROSCOPY
Title (de)
HOCHAUFLÖSENDE 3D-FLUORESZENZMIKROSKOPIE
Title (fr)
MICROSCOPIE DE FLUORESCENCE 3D HAUTE RÉSOLUTION
Publication
Application
Priority
- DE 102013216124 A 20130814
- EP 2014065501 W 20140718
Abstract (en)
[origin: WO2015022146A1] The invention relates to a method of microscopy for generating a high-resolution image (/f) of a sample (2) comprising the following steps: a) providing the sample (2) with a substance which emits specific statistically blinking fluorescent radiation after excitation, or using a sample (2) which contains such a substance, b) directing illuminating radiation (10) onto the sample (2) and thereby exciting the sample (2) for emission of the fluorescent radiation, c) repeating imaging of the sample (2) which emits the fluorescent radiation along an optical axis (OA) onto a spatially resolving detector (5), such that a sequence of images (In) is obtained, d) processing the sequence of images (In) by means of a cumulant function, which evaluates intensity fluctuations in the sequence of images (In) caused by the blinking, and thereby generating an image (If) of a local distribution of the substance in the sample (2) which has a spatial resolution increased beyond the optical resolution of the image, wherein e) directing of the illuminating radiation (10) takes place such that the illuminating radiation (10) excites the sample (2) along the optical axis (OA) only in a limited depth region for emission of the fluorescent radiation.
IPC 8 full level
G02B 21/16 (2006.01); G02B 21/36 (2006.01); G02B 27/58 (2006.01)
CPC (source: EP US)
G01N 21/6458 (2013.01 - US); G02B 21/06 (2013.01 - US); G02B 21/16 (2013.01 - EP US); G02B 21/365 (2013.01 - US); G02B 21/367 (2013.01 - EP US); G02B 27/58 (2013.01 - EP US)
Citation (search report)
See references of WO 2015022146A1
Citation (examination)
DE 102008009216 A1 20090820 - ZEISS CARL MICROIMAGING GMBH [DE]
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
Designated extension state (EPC)
BA ME
DOCDB simple family (publication)
WO 2015022146 A1 20150219; CN 105452931 A 20160330; CN 105452931 B 20180911; DE 102013216124 A1 20150219; EP 3033647 A1 20160622; JP 2016534395 A 20161104; US 2016195704 A1 20160707
DOCDB simple family (application)
EP 2014065501 W 20140718; CN 201480043416 A 20140718; DE 102013216124 A 20130814; EP 14739846 A 20140718; JP 2016533861 A 20140718; US 201414911819 A 20140718