EP 3037807 A1 20160629 - METHOD AND SYSTEM FOR DETECTING A TARGET WITHIN A POPULATION OF MOLECULES
Title (en)
METHOD AND SYSTEM FOR DETECTING A TARGET WITHIN A POPULATION OF MOLECULES
Title (de)
VERFAHREN UND SYSTEM ZUR ERKENNUNG EINES ZIELS IN EINER POPULATION VON MOLEKÜLEN
Title (fr)
PROCÉDÉ ET SYSTÈME POUR DÉTECTER UNE CIBLE DANS UNE POPULATION DE MOLÉCULES
Publication
Application
Previously filed application
PCT/IL2007/001287 20071025 WO
Priority
- US 85472206 P 20061027
- EP 07827261 A 20071025
- IL 2007001287 W 20071025
Abstract (en)
A method of detecting the presence of target molecules within a population of molecules, the method comprising: (a) contacting a plurality of magnetic particles with the population of molecules potentially containing the target molecules, wherein the target molecules bind to the magnetic particles; (b) directing an excitation energy beam into the population of molecules; (c) creating a magnetic field gradient using two electromagnetic poles (312, 314) driven in a cyclical oscillating manner, whereby the magnetic field gradient attracts the magnetic particles and forms a cloud of condensed target molecules with bound magnetic particles, wherein the magnetic field gradient causes the cloud of condensed target molecules with bound magnetic particles to cross a path of the excitation energy beam as the cloud of condensed target molecules with bound magnetic particles crosses between the electromagnetic poles (312, 314) in a cyclical manner; and (d) detecting emission from said cloud of condensed target molecules with bound magnetic particles as it crosses the path of the excitation energy beam. The target comprises one or more molecule types selected from the group consisting of a nucleic acid sequence, an amino acid sequence, a carbohydrate sequence, an ion and a feature of a protein determined by non-primary structure. The probe specific signal may be a fluorescent signal.
IPC 8 full level
G01N 21/64 (2006.01); G01N 33/543 (2006.01)
CPC (source: EP US)
C12Q 1/6818 (2013.01 - US); G01N 21/1717 (2013.01 - EP US); G01N 21/64 (2013.01 - US); G01N 21/6428 (2013.01 - EP US); G01N 33/54326 (2013.01 - US); G01N 33/54333 (2013.01 - EP US); G01N 33/54353 (2013.01 - US); G01N 33/582 (2013.01 - US); G01N 2021/6439 (2013.01 - EP US)
Citation (applicant)
- US 7090995 B2 20060815 - CABANTCHIK IOAV ZVI [IL], et al
- WABUYELE ET AL., JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 125, 2003, pages 6937 - 6945
- CASTRO ET AL., ANALYTICAL CHEMISTRY, vol. 69, 1997, pages 3915 - 3920
- GAU ET AL., BIOSENSORS AND BIOELECTRONICS, vol. 16, 2001, pages 745 - 755
- STORHOFF ET AL., NATURE BIOTECHNOLOGY, vol. 22, 2004, pages 883 - 887
- KATSURA ET AL., SUPERCONDUCTOR SCIENCE AND TECHNOLOGY, vol. 14, 2001, pages 1131 - 1134
Citation (search report)
- [I] US 2004058458 A1 20040325 - ANKER JEFFREY [US], et al
- [A] ANKER JEFFREY N ET AL: "Magnetically modulated optical nanoprobes", APPLIED PHYSICS LETTERS, A I P PUBLISHING LLC, US, vol. 82, no. 7, 17 February 2003 (2003-02-17), pages 1102 - 1104, XP012034812, ISSN: 0003-6951, DOI: 10.1063/1.1544435
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LI LT LU LV MC MT NL PL PT RO SE SI SK TR
DOCDB simple family (publication)
WO 2008050335 A2 20080502; WO 2008050335 A3 20080703; EP 2092309 A2 20090826; EP 3037807 A1 20160629; EP 3037807 B1 20210331; US 10175246 B2 20190108; US 11275083 B2 20220315; US 2010041166 A1 20100218; US 2013274137 A1 20131017; US 2017138952 A1 20170518; US 2019137504 A1 20190509; US 8465989 B2 20130618; US 9575068 B2 20170221
DOCDB simple family (application)
IL 2007001287 W 20071025; EP 07827261 A 20071025; EP 15201074 A 20071025; US 201313909109 A 20130604; US 201715420159 A 20170131; US 201916240839 A 20190107; US 31207107 A 20071025