EP 3052624 A1 20160810 - SYSTEMATIC OPTIMIZATION OF CODING SEQUENCE FOR FUNCTIONAL PROTEIN EXPRESSION
Title (en)
SYSTEMATIC OPTIMIZATION OF CODING SEQUENCE FOR FUNCTIONAL PROTEIN EXPRESSION
Title (de)
SYSTEMATISCHE OPTIMIERUNG VON CODIERUNGSSEQUENZEN FÜR EINE FUNKTIONELLE PROTEINEXPRESSION
Title (fr)
OPTIMISATION SYSTÉMATIQUE DE LA SÉQUENCE CODANT POUR L'EXPRESSION FONCTIONNELLE D'UNE PROTÉINE
Publication
Application
Priority
EP 2013070531 W 20131002
Abstract (en)
[origin: WO2015048989A1] The present invention relates to a systematic approach to codon selection for provision of a coding sequence for functional expression of a heterologous protein in a host cell. This approach recognizes that non-optimal codons for translation efficiency may be desirable at certain sites and relies on the provision of an expression library of synonymous coding sequences for the protein of concern. Expression of each such sequence is compared under the same conditions in the host cell, desirably by high–throughput screening.
IPC 8 full level
C12N 15/09 (2006.01)
CPC (source: EP)
C12N 15/67 (2013.01)
Citation (search report)
See references of WO 2015048989A1
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
Designated extension state (EPC)
BA ME
DOCDB simple family (publication)
DOCDB simple family (application)
EP 2013070531 W 20131002; EP 13773223 A 20131002