EP 3057975 B1 20190904 - N-GLYCOSYLATION OF PEPTIDES AND PROTEINS
Title (en)
N-GLYCOSYLATION OF PEPTIDES AND PROTEINS
Title (de)
N-GLYCOSYLIERUNG VON PEPTIDES UND PROTEINES
Title (fr)
N-GLYCOSYLATION DEPEPTIDES ET DE PROTEINES
Publication
Application
Priority
- GB 201318313 A 20131016
- GB 201321911 A 20131211
- GB 2014053099 W 20141015
Abstract (en)
[origin: WO2015056011A1] A process for the production of a glycoconjugate by N-glycosylation of a protein or peptide comprising the sequence D/E-X-N-X-S/T, wherein each X is the same or different and is any natural amino acid other than proline, wherein the process comprises reacting the protein or peptide with a polyisoprenyl pyrophosphate of formula (I), or a salt thereof, in the presence of PglB: (I) to produce the glycoconjugate comprising the protein or peptide having a saccharide [SI] linked to the asparagine in the sequence D/E-X-N-X-S/T. Polyisoprenylpyrophosphates used as substrates in the biocatalytic process are also provided, as well as certain glycoconjugates.
IPC 8 full level
C07H 11/04 (2006.01); C07K 9/00 (2006.01); C12P 19/18 (2006.01)
CPC (source: EP US)
C07H 11/04 (2013.01 - EP US); C07K 9/00 (2013.01 - EP US); C12P 19/18 (2013.01 - EP US); C12P 19/28 (2013.01 - EP US)
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
DOCDB simple family (publication)
WO 2015056011 A1 20150423; EP 3057975 A1 20160824; EP 3057975 B1 20190904; US 2016257983 A1 20160908; US 2022025419 A1 20220127
DOCDB simple family (application)
GB 2014053099 W 20141015; EP 14787034 A 20141015; US 201415029730 A 20141015; US 202117190128 A 20210302