EP 3060683 A4 20170809 - METHODS AND APPARATUS FOR POINT-OF-CARE NUCLEIC ACID AMPLIFICATION AND DETECTION
Title (en)
METHODS AND APPARATUS FOR POINT-OF-CARE NUCLEIC ACID AMPLIFICATION AND DETECTION
Title (de)
VERFAHREN UND VORRICHTUNG FÜR NUKLEINSÄUREAMPLIFIKATION UND -NACHWEIS AM VERSORGUNGSORT
Title (fr)
PROCÉDÉS ET APPAREIL PERMETTANT L'AMPLIFICATION ET LA DÉTECTION D'ACIDES NUCLÉIQUES AU POINT D'INTERVENTION
Publication
Application
Priority
- US 201361894392 P 20131022
- US 201414262683 A 20140425
- US 2014061808 W 20141022
Abstract (en)
[origin: WO2015061480A1] Methods and apparatus are provided for point-of-care nucleic acid amplification and detection. One embodiment of the invention comprises a fully integrated, sample-to-answer molecular diagnostic instrument that optionally may be used in a multiplexed fashion to detect multiple target nucleic acid sequences of interest and that optionally may be configured for disposal after one-time use. The instrument preferable utilizes an isothermal nucleic acid amplification technique, such as loop-mediated isothermal amplification (LAMP), to reduce the instrumentation requirements associated with nucleic acid amplification. Detection of target amplification may be achieved, for example, via detection of a color shift or fluorescence in a dye added to the amplification reaction. Such detection may be performed visually by an operator or may be achieved utilizing an imaging technique, e.g., spectrophotometric imaging.
IPC 8 full level
CPC (source: EP)
B01L 3/502715 (2013.01); B01L 3/502738 (2013.01); B01L 3/523 (2013.01); C12Q 1/6846 (2013.01); B01L 7/52 (2013.01); B01L 2200/16 (2013.01); B01L 2300/044 (2013.01); B01L 2300/0672 (2013.01); B01L 2300/0816 (2013.01); B01L 2300/0864 (2013.01); B01L 2300/0867 (2013.01); B01L 2300/0874 (2013.01); B01L 2300/1827 (2013.01); B01L 2400/0487 (2013.01); B01L 2400/0616 (2013.01); B01L 2400/065 (2013.01)
Citation (search report)
- [Y] WO 2007011867 A2 20070125 - APPLERA CORP [US], et al
- [Y] WO 2006018044 A1 20060223 - AGILENT TECHNOLOGIES INC [US], et al
- [A] WO 2011097412 A1 20110811 - HARVARD COLLEGE [US], et al
- See references of WO 2015061480A1
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
DOCDB simple family (publication)
WO 2015061480 A1 20150430; CA 2928406 A1 20150430; CN 105874084 A 20160817; EP 3060683 A1 20160831; EP 3060683 A4 20170809; JP 2016539633 A 20161222
DOCDB simple family (application)
US 2014061808 W 20141022; CA 2928406 A 20141022; CN 201480070428 A 20141022; EP 14856081 A 20141022; JP 2016526159 A 20141022