EP 3087380 A1 20161102 - METHOD FOR DETERMINING THE CELL AGGRESSIVENESS GRADE OF CANCER CELLS OR OF CANCER STEM CELLS
Title (en)
METHOD FOR DETERMINING THE CELL AGGRESSIVENESS GRADE OF CANCER CELLS OR OF CANCER STEM CELLS
Title (de)
VERFAHREN ZUR BESTIMMUNG DES AGGRESSIVITÄTSGRADES VON KREBSZELLEN ODER KREBSSTAMMZELLEN
Title (fr)
PROCÉDÉ DE DÉTERMINATION DU GRADE D'AGRESSIVITE CELLULAIRE DE CELLULES CANCEREUSES OU DE CELLULES SOUCHES CANCEREUSES
Publication
Application
Priority
- FR 1363547 A 20131224
- FR 2014053552 W 20141224
Abstract (en)
[origin: WO2015097419A1] The subject matter of the invention is a method for determining, in vitro, the cell aggressiveness grade of cancer cells or for detecting cancer stem cells in a cell sample originating from a solid tissue suspected of being cancerous, comprising at least the following steps: a. dissociation of the cell cluster constituting the sample into a suspension of whole and viable isolated cells, b. macroscopic sorting of the cells so as to obtain homogeneous subpopulations, c. calibration of at least one microwave electromagnetic sensor resonating at its own resonance frequency, d. presentation of the cells dissociated and sorted according to steps a. and b. on the at least one previously calibrated sensor, e. interrogation of the at least one sensor and determination of the new resonance frequency of said at least one sensor having received the cells, f. calculation of the variation in overall dielectric permittivity of the cells according to the variation in working frequency, which constitutes the electromagnetic signature of said cells. The macroscopic sorting is without prior labelling and is based on the intrinsic properties of the cells. The invention also covers a kit suitable for implementing the method.
IPC 8 full level
G01N 27/22 (2006.01); G01N 22/00 (2006.01)
CPC (source: EP US)
G01N 22/00 (2013.01 - EP US); G01N 27/221 (2013.01 - US); G01N 33/4833 (2013.01 - EP US)
Citation (search report)
See references of WO 2015097419A1
Citation (examination)
- ZHANG LING YAN ET AL: "Label-free colorectal cancer cell line bio-sensing using RF resonator", 2013 TRANSDUCERS & EUROSENSORS XXVII: THE 17TH INTERNATIONAL CONFERENCE ON SOLID-STATE SENSORS, ACTUATORS AND MICROSYSTEMS (TRANSDUCERS & EUROSENSORS XXVII), IEEE, 16 June 2013 (2013-06-16), pages 1194 - 1197, XP032499461, DOI: 10.1109/TRANSDUCERS.2013.6626987
- MÉLIN CAROLE ET AL: "Cancer Stem Cell Sorting from Colorectal Cancer Cell Lines by Sedimentation Field Flow Fractionation", ANALYTICAL CHEMISTRY, vol. 84, no. 3, 7 February 2012 (2012-02-07), US, pages 1549 - 1556, XP055800406, ISSN: 0003-2700, Retrieved from the Internet <URL:https://pubs.acs.org/doi/pdf/10.1021/ac202797z> DOI: 10.1021/ac202797z
- VINCENT PETIT ET AL: "Optimization of tumor xenograft dissociation for the profiling of cell surface markers and nutrient transporters", LABORATORY INVESTIGATION, vol. 93, no. 5, 4 March 2013 (2013-03-04), pages 611 - 621, XP055155682, ISSN: 0023-6837, DOI: 10.1038/labinvest.2013.44
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
Designated extension state (EPC)
BA ME
DOCDB simple family (publication)
FR 3015521 A1 20150626; FR 3015521 B1 20210528; EP 3087380 A1 20161102; US 2016320316 A1 20161103; WO 2015097419 A1 20150702
DOCDB simple family (application)
FR 1363547 A 20131224; EP 14831029 A 20141224; FR 2014053552 W 20141224; US 201415107831 A 20141224