EP 3092495 A4 20180103 - SRM ASSAY FOR PD-L1
Title (en)
SRM ASSAY FOR PD-L1
Title (de)
SRM-TEST AUF PD-L1
Title (fr)
DOSAGE SRM POUR PD-L1
Publication
Application
Priority
- US 201461924218 P 20140106
- US 2015010386 W 20150106
Abstract (en)
[origin: WO2015103645A2] The current disclosure provides for specific peptides, and derived ionization characteristics of the peptides, from the PD-L1 protein that are particularly advantageous for quantifying the PD-L1 protein directly in biological samples that have been fixed in formalin by the method of Selected Reaction Monitoring (SRM) mass spectrometry, or what can also be termed as Multiple Reaction Monitoring (MRM) mass spectrometry. Such biological samples are chemically preserved and fixed wherein said biological sample is selected from tissues and cells treated with formaldehyde containing agents/fixatives including formalin-fixed tissue/cells, formalin-fixed/paraffin embedded (FFPE) tissue/cells, FFPE tissue blocks and cells from those blocks, and tissue culture cells that have been formalin fixed and or paraffin embedded. A protein sample is prepared from said biological sample using the Liquid Tissue™ reagents and protocol and the PD-Ll protein are quantitated in the Liquid Tissue™ sample by the method of SRM/MRM mass spectrometry by quantitating in the protein sample at least one or more of the peptides described. These peptides can be quantitated if they reside in a modified or an unmodified form. An example of a modified form of a PD-Ll fragment peptide is phosphorylation of a tyrosine, threonine, serine, and/or other amino acid residues within the peptide sequence.
IPC 8 full level
G01N 33/68 (2006.01); C07K 7/04 (2006.01); C07K 16/28 (2006.01)
CPC (source: EP US)
C07K 16/2827 (2013.01 - US); G01N 33/57423 (2013.01 - EP US); G01N 33/6848 (2013.01 - EP US); A61K 2039/507 (2013.01 - US); G01N 2333/70596 (2013.01 - US)
Citation (search report)
- [X] JP 2012197258 A 20121018 - UNIV TOHOKU
- See references of WO 2015103645A2
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
DOCDB simple family (publication)
WO 2015103645 A2 20150709; WO 2015103645 A3 20150911; AU 2015203904 A1 20160804; AU 2015203904 B2 20180719; CA 2936077 A1 20150709; CA 2936077 C 20200630; CN 106164676 A 20161123; CN 106164676 B 20190705; EP 3092495 A2 20161116; EP 3092495 A4 20180103; EP 3092495 B1 20201125; IL 246642 A0 20160831; JP 2017503503 A 20170202; JP 2019058171 A 20190418; JP 6612414 B2 20191127; US 2016313343 A1 20161027
DOCDB simple family (application)
US 2015010386 W 20150106; AU 2015203904 A 20150106; CA 2936077 A 20150106; CN 201580011369 A 20150106; EP 15733236 A 20150106; IL 24664216 A 20160706; JP 2016544870 A 20150106; JP 2018203065 A 20181029; US 201615203704 A 20160706