Global Patent Index - EP 3095858 A1

EP 3095858 A1 20161123 - THERMOSTABLE GLYCOSIDE HYDROLASE

Title (en)

THERMOSTABLE GLYCOSIDE HYDROLASE

Title (de)

THERMOSTABILE GLYKOSIDHYDROLASE

Title (fr)

GLYCOSIDE HYDROLASE THERMOSTABLE

Publication

EP 3095858 A1 20161123 (EN)

Application

EP 16170320 A 20160519

Priority

JP 2015101769 A 20150519

Abstract (en)

A thermostable glycoside hydrolase having a glycoside hydrolase catalytic domain including: (A) a polypeptide including the amino acid sequence represented by SEQ ID NO: 1; (B) a polypeptide including an amino acid sequence in which at least one amino acid has been deleted, substituted, or added in the amino acid sequence represented by SEQ ID NO: 1, and having hydrolysis activity against a substrate of PSA and hydrolysis activity against a substrate of CMC at least under conditions of 65°C and pH 4.0; (C) a polypeptide including an amino acid sequence having 80% or greater sequence identity with the amino acid sequence represented by SEQ ID NO: 1, and having hydrolysis activity against a substrate of PSA and hydrolysis activity against a substrate of CMC at least under conditions of 65°C and pH 4.0; (D) a polypeptide in which are linked, either directly or via a linker, a polypeptide including an amino acid sequence in which at least one amino acid has been deleted, substituted, or added in the region composed of amino acids from positions 51 to 485 in the amino acid sequence represented by SEQ ID NO: 1, and having hydrolysis activity against a substrate of PSA at least under conditions of 65°C and pH 4.0, and a polypeptide including an amino acid sequence in which at least one amino acid has been deleted, substituted, or added in the region composed of amino acids from positions 530 to 823 in the amino acid sequence represented by SEQ ID NO: 1, and having hydrolysis activity against a substrate of CMC at least under conditions of 65°C and pH 4.0; or (E) a polypeptide in which are linked, either directly or via a linker, a polypeptide including an amino acid sequence having 80% or greater sequence identity with the region composed of amino acids from position 51 to position 485 in the amino acid sequence represented by SEQ ID NO: 1 and having hydrolysis activity against a substrate of PSA at least under conditions of 65°C and pH 4.0, and a polypeptide including an amino acid sequence having 80% or greater sequence identity with the region composed of amino acids from positions 530 to 823 in the amino acid sequence represented by SEQ ID NO: 1 and having hydrolysis activity against a substrate of CMC at least under conditions of 65°C and pH 4.0.

IPC 8 full level

C12N 9/42 (2006.01)

CPC (source: EP US)

C12N 9/2437 (2013.01 - EP US); C12P 19/02 (2013.01 - US); C12P 19/12 (2013.01 - US); C12P 19/14 (2013.01 - US); C12Y 302/01004 (2013.01 - EP US)

Citation (applicant)

  • WO 2014157492 A1 20141002 - HONDA MOTOR CO LTD [JP], et al
  • US 2003054539 A1 20030320 - SCHULEIN MARTIN [DK], et al
  • US 2012135499 A1 20120531 - BOWER BENJAMIN S [US], et al
  • YI ET AL., PLOSONE, vol. 8, 2013, pages E84172
  • ZOU ET AL., MICROBIAL CELL FACTORIES, vol. 11, 2012, pages 21
  • QUINLAN ET AL., NATURE METHODS, vol. 5, 2008, pages 179 - 181
  • NOGUCHI ET AL.: "MetaGeneAnnotator: Detecting Species-Specific Patterns of Ribosomal Binding Site for Precise Gene Prediction in Anonymous Prokaryotic and Phage Genomes", DNA RES., vol. 15, 2008, pages 387 - 396
  • FINN ET AL., NUCLEIC ACIDS RESEARCH DATABASE, 2010, pages 211 - 222
  • DURBIN ET AL.: "The theory behind profile HMMs. Biological sequence analysis: probabilistic models of proteins and nucleic acids", 1998, CAMBRIDGE UNIVERSITY PRESS;

Citation (search report)

Designated contracting state (EPC)

AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

Designated extension state (EPC)

BA ME

DOCDB simple family (publication)

EP 3095858 A1 20161123; EP 3095858 B1 20190102; JP 2016214128 A 20161222; JP 6586659 B2 20191009; US 10006013 B2 20180626; US 2016340663 A1 20161124

DOCDB simple family (application)

EP 16170320 A 20160519; JP 2015101769 A 20150519; US 201615156759 A 20160517