EP 3102677 A1 20161214 - GENOMIC DNA EXTRACTION REAGENT AND METHOD
Title (en)
GENOMIC DNA EXTRACTION REAGENT AND METHOD
Title (de)
GENOMISCHES DNA-EXTRAKTIONSREAGENZ UND -VERFAHREN
Title (fr)
RÉACTIF D'EXTRACTION D'ADN GÉNOMIQUE ET PROCÉDÉ AFFÉRENT
Publication
Application
Priority
- US 201414171816 A 20140204
- US 201414211113 A 20140314
- US 2014072151 W 20141223
Abstract (en)
[origin: WO2015119719A1] The present invention is directed to a genomic DNA extraction reagent and method for improved extraction of DNA from biological tissue. The extraction reagent of the invention is mixed with disrupted biological tissue to form a DNA extraction solute which is incubated in a DNA extraction step. The extraction reagent includes an alkali component to maintain the DNA extraction solute at a pH of about 10 to 14 substantially throughout the extraction step. The extraction solute is centrifuged to clarify the supernatant. The supernatant containing the extracted DNA is diluted with a neutralizing buffer resulting in a high throughout method of generating high quantities of high quality DNA. Major PCR inhibitors are managed with the unique chemical combinations of the DNA extraction reagent designed and optimized for extraction of DNA from plant tissue and cells.
IPC 8 full level
C12N 15/10 (2006.01)
CPC (source: EP)
C12N 15/1003 (2013.01)
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
Designated extension state (EPC)
BA ME
DOCDB simple family (publication)
WO 2015119719 A1 20150813; AU 2014381626 A1 20150910; CA 2901406 A1 20150813; EP 3102677 A1 20161214; EP 3102677 A4 20170628
DOCDB simple family (application)
US 2014072151 W 20141223; AU 2014381626 A 20141223; CA 2901406 A 20141223; EP 14881909 A 20141223