EP 3134505 A1 20170301 - METHOD AND APPARATUS FOR PURIFYING BIOLOGICAL MOLECULES
Title (en)
METHOD AND APPARATUS FOR PURIFYING BIOLOGICAL MOLECULES
Title (de)
VERFAHREN UND VORRICHTUNG ZUR AUFREINIGUNG VON BIOLOGISCHEN MOLEKÜLEN
Title (fr)
PROCÉDÉ ET DISPOSITIF DE PURIFICATION DE MOLÉCULES BIOLOGIQUES
Publication
Application
Priority
- DE 102014207774 A 20140425
- EP 2015058348 W 20150417
Abstract (en)
[origin: WO2015162059A1] In a method and apparatus for purifying biological molecules, especially nucleic acids or proteins, at least one filter (50) is used. At least some of the fluids needed for the operation are pumped in a circuit (51) via the filter (50). First a fluid with biological cells is pumped via the filter (50). The cells retained on the filter are digested. To bind the biological molecules to the filter, binding buffer is pumped in the circuit (51) via the filter (50). A washing buffer for cleaning the biological molecules bound to the filter is pumped via the filter (50), and so the biological molecules bound to the filter are available for further use.
IPC 8 full level
C12N 1/06 (2006.01); B01D 61/14 (2006.01); C12M 1/00 (2006.01); C12N 15/10 (2006.01)
CPC (source: CN EP KR US)
C07K 1/34 (2013.01 - KR); C12M 23/16 (2013.01 - KR); C12M 47/02 (2013.01 - CN EP KR US); C12M 47/06 (2013.01 - CN EP KR US); C12M 47/10 (2013.01 - US); C12M 47/12 (2013.01 - CN EP KR US); C12N 1/06 (2013.01 - CN EP KR US); C12N 15/1017 (2013.01 - KR)
Citation (search report)
See references of WO 2015162059A1
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
Designated extension state (EPC)
BA ME
DOCDB simple family (publication)
WO 2015162059 A1 20151029; CN 106232799 A 20161214; DE 102014207774 A1 20151029; DE 102014207774 B4 20151231; EP 3134505 A1 20170301; JP 2017515500 A 20170615; KR 20160145610 A 20161220; US 2017044483 A1 20170216
DOCDB simple family (application)
EP 2015058348 W 20150417; CN 201580021197 A 20150417; DE 102014207774 A 20140425; EP 15717482 A 20150417; JP 2017507070 A 20150417; KR 20167029554 A 20150417; US 201515305172 A 20150417