EP 3137485 A1 20170308 - CHELATOR MODIFIED MAGNETIC SILICA NANOPARTICLES, THEIR USE AND PREPARATION
Title (en)
CHELATOR MODIFIED MAGNETIC SILICA NANOPARTICLES, THEIR USE AND PREPARATION
Title (de)
CHELATMODIFIZIERTE MAGNETISCHE SILICIUMDIOXIDPARTIKEL, DEREN VERWENDUNG UND HERSTELLUNG
Title (fr)
NANOPARTICULES CHÉLATRICES DE SILICE MAGNÉTIQUE MODIFIÉE, LEUR UTILISATION ET LEUR PRÉPARATION
Publication
Application
Priority
- PT 10760814 A 20140428
- IB 2015053093 W 20150428
Abstract (en)
[origin: WO2015166415A1] The present solution relates to magnetic probes/nanoprobes (NP) for the selective enrichment of proteins. Namely, the solution relates to magnetic nanoparticles, which surface is chemically modified with metal ion chelation groups. The use of these magnetic nanoparticles allows the separation of specific proteins from complex mixtures by means of a magnetic field, namely proteins containing metal ions in their composition, such as metalloproteases, and the recovery of metal binding proteins, such as histidine-tagged recombinant proteins. This solution describes a probe for select metalloproteins in a solution comprising at least an inorganic magnetic core particle comprising a paramagnetic, superparamagnetic or ferromagnetic material; wherein such particle is coated with a siliceous coating; wherein the siliceous coating further comprises a plurality of metal ion chelating moieties; wherein the size of the probe is less than 1100 nm. Furthermore, this disclosure also describes a method for producing said probe.
IPC 8 full level
C07K 1/22 (2006.01); B01D 15/38 (2006.01); B01J 13/18 (2006.01); B01J 20/32 (2006.01); B01J 45/00 (2006.01); B22F 1/102 (2022.01); B22F 1/16 (2022.01); B22F 9/24 (2006.01); H01F 1/00 (2006.01); B22F 1/054 (2022.01)
CPC (source: EP US)
B01D 15/3828 (2013.01 - EP); B01J 13/18 (2013.01 - EP); B01J 20/103 (2013.01 - EP); B01J 20/28009 (2013.01 - EP); B01J 20/3204 (2013.01 - EP); B01J 20/3242 (2013.01 - EP); B01J 20/3259 (2013.01 - EP); B01J 20/3293 (2013.01 - EP); B22F 1/102 (2022.01 - EP US); B22F 1/16 (2022.01 - EP US); C07K 1/22 (2013.01 - EP US); H01F 1/0054 (2013.01 - EP); B22F 1/054 (2022.01 - EP US); B22F 1/056 (2022.01 - EP US); B82Y 30/00 (2013.01 - EP); B82Y 40/00 (2013.01 - EP); C07K 2319/21 (2013.01 - EP); C22C 2202/02 (2013.01 - EP)
Citation (search report)
See references of WO 2015166415A1
Citation (examination)
- CN 102091575 A 20110615 - SHENZHEN INST OF ADV TECH CAS
- CN 102815753 A 20121212 - UNIV SHANGHAI JIAOTONG
- WO 2014200790 A1 20141218 - 3M INNOVATIVE PROPERTIES CO [US]
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
Designated extension state (EPC)
BA ME
DOCDB simple family (publication)
DOCDB simple family (application)
IB 2015053093 W 20150428; EP 15727465 A 20150428