Global Patent Index - EP 3147362 A1

EP 3147362 A1 20170329 - MULTIFUNCTIONAL ALLELES

Title (en)

MULTIFUNCTIONAL ALLELES

Title (de)

MULTIFUNKTIONS-ALLELE

Title (fr)

ALLÈLES MULTIFONCTIONNELLES

Publication

EP 3147362 A1 20170329 (EN)

Application

EP 16196614 A 20101029

Priority

  • US 25607809 P 20091029
  • EP 10774391 A 20101029
  • US 2010054654 W 20101029

Abstract (en)

Nucleic acid constructs and methods for rendering modifications to a genome are provided, wherein the modifications comprise null alleles, conditional alleles and null alleles comprising COINS. Multifunctional alleles (MFA) are provided, as well as methods for making them, which afford the ability in a single targeting to introduce an allele that can be used to generate a null allele, a conditional allele, or an allele that is a null allele and that further includes a COIN. MFAs comprise pairs of cognate recombinase recognition sites, an actuating sequence and/or a drug selection cassette, and a nucleotide sequence of interest, and a COIN, wherein upon action of a recombinase a conditional allele with a COIN is formed. In a further embodiment, action of a second recombinase forms an allele that contains only a COIN in sense orientation. In a further embodiment, action by a third recombinase forms an allele that contains only the actuating sequence in sense orientation.

IPC 8 full level

C12N 15/10 (2006.01); C12N 15/90 (2006.01)

CPC (source: CN EP US)

C12N 9/00 (2013.01 - CN EP US); C12N 15/63 (2013.01 - EP US); C12N 15/907 (2013.01 - CN EP US); A01K 2217/15 (2013.01 - CN EP US); A01K 2217/20 (2013.01 - CN EP US); C12N 2800/30 (2013.01 - CN EP US); C12N 2800/60 (2013.01 - CN EP US); C12N 2840/44 (2013.01 - CN EP US)

Citation (applicant)

  • US 7473557 B2 20090106 - ECONOMIDES ARIS N [US], et al
  • US 7205148 B2 20070417 - ECONOMIDES ARIS N [US], et al
  • US 6586251 B2 20030701 - ECONOMIDES ARIS N [US], et al
  • VALENZUELA ET AL.: "High-throughput engineering of the mouse genome coupled with high-resolution expression analysis", NATURE BIOTECH., vol. 21, no. 6, 2003, pages 652 - 659
  • DOETSCHMAN, T. ET AL.: "Targeted correction of mutant HPRT gene in mouse embryonic stem cells", NATURE, vol. 330, 1987, pages 576 - 578, XP001157293, DOI: doi:10.1038/330576a0
  • RAYMOND, C.S.; SORIANO, P.: "High-efficiency FLP and PhiC31 site-specific recombination in mammalian cells", PLOS ONE, vol. 2, 2007, pages E162
  • SAUER, B.; HENDERSON, N.: "Site-specific DNA recombination in mammalian cells by the Cre recombinase of bacteriophage P1", PROC. NATL. ACAD. SCI. USA, vol. 85, 1988, pages 5166 - 5170, XP000615552, DOI: doi:10.1073/pnas.85.14.5166

Citation (search report)

  • [A] WO 02088353 A2 20021107 - ADEREGEM [FR], et al
  • [A] EP 1662005 A1 20060531 - FRANKGEN BIOTECHNOLOGIE AG [DE], et al
  • [A] WO 2006056617 A1 20060601 - FRANKGEN BIOTECHNOLOGIE AG [DE], et al
  • [A] WO 0240685 A2 20020523 - CORNELL RES FOUNDATION INC [US], et al
  • [A] WO 2005001087 A2 20050106 - REGENERON PHARMA [US], et al
  • [A] US 2006064769 A1 20060323 - JONES STEPHEN [US], et al
  • [A] WO 0236789 A2 20020510 - REGENERON PHARMA [US], et al
  • [A] CRUZ J JR ET AL M ET AL: "Conditionals by inversion ( COINs): a high-throughput method for engineering conditional alleles & hprt as a test locus", TRANSGENIC RESEARCH, vol. 14, no. 4, August 2005 (2005-08-01), & 6TH TRANSGENIC TECHNOLOGY MEETING; BARCELONA, SPAIN; SEPTEMBER 11 -13, 2005, pages 520 - 521, XP002615214, ISSN: 0962-8819
  • [AD] VALENZUELA D M ET AL: "High-throughput engineering of the mouse genome coupled with high-resolution expression analysis", NATURE BIOTECHNOLOGY 20030601 PRESSE DIENSTLEISTUNGSGESELLSCHAFT MBH UND CO. KG US, vol. 21, no. 6, 1 June 2003 (2003-06-01), pages 652 - 659, XP002615215, DOI: 10.1038/NBT822
  • [T] A. N. ECONOMIDES ET AL: "Conditionals by inversion provide a universal method for the generation of conditional alleles", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, vol. 110, no. 34, 5 August 2013 (2013-08-05), pages E3179 - E3188, XP055155451, ISSN: 0027-8424, DOI: 10.1073/pnas.1217812110

Designated contracting state (EPC)

AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

DOCDB simple family (publication)

US 2011104799 A1 20110505; AU 2010319894 A1 20120531; AU 2010319894 B2 20150305; CA 2779858 A1 20110519; CA 2779858 C 20191029; CN 102666854 A 20120912; CN 102666854 B 20160824; CN 106191126 A 20161207; CN 106191126 B 20200414; CY 1118659 T1 20170712; CY 1121457 T1 20200529; DK 2494047 T3 20170410; DK 3147362 T3 20190408; EP 2494047 A1 20120905; EP 2494047 B1 20170104; EP 3147362 A1 20170329; EP 3147362 B1 20190102; ES 2613662 T3 20170525; ES 2716981 T3 20190618; JP 2013509192 A 20130314; JP 2016063839 A 20160428; JP 2016185166 A 20161027; JP 5908405 B2 20160426; JP 6085696 B2 20170222; JP 6333316 B2 20180530; PL 2494047 T3 20170531; PL 3147362 T3 20190628; PT 2494047 T 20170208; PT 3147362 T 20190402; TR 201903376 T4 20190422; US 10392633 B2 20190827; US 11319557 B2 20220503; US 2013302899 A1 20131114; US 2019390230 A1 20191226; US 2022220510 A1 20220714; WO 2011059799 A1 20110519

DOCDB simple family (application)

US 91544710 A 20101029; AU 2010319894 A 20101029; CA 2779858 A 20101029; CN 201080056458 A 20101029; CN 201610565668 A 20101029; CY 171100118 T 20170126; CY 191100345 T 20190326; DK 10774391 T 20101029; DK 16196614 T 20101029; EP 10774391 A 20101029; EP 16196614 A 20101029; ES 10774391 T 20101029; ES 16196614 T 20101029; JP 2012537099 A 20101029; JP 2016017198 A 20160201; JP 2016148272 A 20160728; PL 10774391 T 20101029; PL 16196614 T 20101029; PT 10774391 T 20101029; PT 16196614 T 20101029; TR 201903376 T 20101029; US 2010054654 W 20101029; US 201313940609 A 20130712; US 201916459236 A 20190701; US 202217712003 A 20220401