EP 3162812 A1 20170503 - MUTANT HEMAGGLUTININ COMPLEX PROTEIN, AND METHOD FOR CULTURING PLURIPOTENT STEM CELLS USING SAME
Title (en)
MUTANT HEMAGGLUTININ COMPLEX PROTEIN, AND METHOD FOR CULTURING PLURIPOTENT STEM CELLS USING SAME
Title (de)
MUTANTES HÄMAGGLUTININKOMPLEXPROTEIN UND VERFAHREN ZUR KULTIVIERUNG VON PLURIPOTENTEN STAMMZELLEN DAMIT
Title (fr)
PROTÉINE COMPLEXE D'HÉMAGGLUTININE MUTANTE ET PROCÉDÉ POUR LA CULTURE DE CELLULES SOUCHES PLURIPOTENTES L'UTILISANT
Publication
Application
Priority
- JP 2014133364 A 20140627
- JP 2015068715 W 20150629
Abstract (en)
Provided are a novel hemagglutinin that can remove cells deviated from the undifferentiated state, the cells being cells that emerge in a colony during culture of stem cells having pluripotency, and a novel method for culturing stem cells having pluripotency, the method using hemagglutinin. Provided is a mutant hemagglutinin complex protein derived from Clostridium botulinum type B, the complex protein containing at least subcomponents HA2 and HA3 of hemagglutinin derived from Clostridium botulinum type B, and the complex protein containing amino acids constituting a glycosylation site, with at least one of the amino acids having been mutated. Provided is a method for culturing stem cells having pluripotency, the method including culturing the stem cell having pluriopotency in the presence of a mutant hemagglutinin complex protein of the present disclosure.
IPC 8 full level
C07K 14/33 (2006.01); C07K 19/00 (2006.01); C12N 1/00 (2006.01); C12N 5/0735 (2010.01); C12N 5/10 (2006.01); C12N 15/09 (2006.01)
CPC (source: EP KR US)
C07K 14/33 (2013.01 - EP KR US); C07K 19/00 (2013.01 - EP US); C12N 1/00 (2013.01 - US); C12N 5/0081 (2013.01 - US); C12N 5/067 (2013.01 - KR); C12N 5/0696 (2013.01 - EP KR US); C12N 5/10 (2013.01 - US); C12N 15/09 (2013.01 - US); C07K 2319/00 (2013.01 - KR); C12N 2500/72 (2013.01 - EP); C12N 2501/727 (2013.01 - EP); C12N 2501/998 (2013.01 - EP US); C12N 2502/14 (2013.01 - KR)
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
Designated extension state (EPC)
BA ME
DOCDB simple family (publication)
EP 3162812 A1 20170503; EP 3162812 A4 20180228; AU 2015281030 A1 20170216; BR 112016030510 A2 20171031; CA 2953670 A1 20151230; CN 106459223 A 20170222; JP 6355736 B2 20180711; JP WO2015199243 A1 20170427; KR 20170020431 A 20170222; SG 11201610878Q A 20170227; US 11230701 B2 20220125; US 2017130206 A1 20170511; WO 2015199243 A1 20151230
DOCDB simple family (application)
EP 15811956 A 20150629; AU 2015281030 A 20150629; BR 112016030510 A 20150629; CA 2953670 A 20150629; CN 201580034073 A 20150629; JP 2015068715 W 20150629; JP 2016529687 A 20150629; KR 20177000761 A 20150629; SG 11201610878Q A 20150629; US 201515321835 A 20150629