EP 3169338 A1 20170524 - METHODS FOR THE INTERCELLULAR TRANSFER OF ISOLATED MITOCHONDRIA IN RECIPIENT CELLS
Title (en)
METHODS FOR THE INTERCELLULAR TRANSFER OF ISOLATED MITOCHONDRIA IN RECIPIENT CELLS
Title (de)
VERFAHREN ZUR INTERZELLULÄREN ÜBERTRAGUNG VON ISOLIERTEN MITOCHONDRIEN IN EMPFÄNGERZELLEN
Title (fr)
PROCÉDÉS DE TRANSFERT INTERCELLULAIRE DE MITOCHONDRIES ISOLÉES DANS DES CELLULES RECEVEUSES
Publication
Application
Priority
- EP 14306154 A 20140716
- EP 2015066190 W 20150715
Abstract (en)
[origin: WO2016008937A1] The present invention relates to methods for the intercellular transfer of isolated mitochondria in recipient cells. In particular, the present invention relates to a method for the intercellular transfer of an amount of mitochondria isolated from a population of donor cells into a population of recipient cells comprising the step of i) centrifuging the population of recipient mammalian cells in presence of the isolated mitochondria at centrifugation force ranging from 1000g to a 2000g at a temperature ranging from 1°C to 8°C and for a time ranging from 5min to 30min, ii) resting the centrifuged cells at a temperature ranging from 30°C to 40°C for a time ranging from 90min to 180min, and iii) repeating the cycling of steps i) and ii) for a sufficient number of times for reaching transfer efficiency.
IPC 8 full level
A61K 35/12 (2015.01); C12N 5/00 (2006.01)
CPC (source: EP US)
A61K 9/0019 (2013.01 - US); A61K 35/28 (2013.01 - EP US); A61K 35/768 (2013.01 - US); A61K 38/193 (2013.01 - EP US); A61K 39/3955 (2013.01 - US); C07K 16/2818 (2013.01 - US); C07K 16/2827 (2013.01 - US); C12N 5/00 (2013.01 - EP US); C12N 7/00 (2013.01 - US); A61K 2039/505 (2013.01 - US); C07K 2317/76 (2013.01 - US); C12N 2710/24132 (2013.01 - US)
Citation (examination)
WO 03046141 A2 20030605 - ADVANCED CELL TECH INC [US], et al
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
Designated extension state (EPC)
BA ME
DOCDB simple family (publication)
DOCDB simple family (application)
EP 2015066190 W 20150715; EP 15738898 A 20150715