EP 3180445 A1 20170621 - MULTIPLEX TRANSCRIPTOME ANALYSIS
Title (en)
MULTIPLEX TRANSCRIPTOME ANALYSIS
Title (de)
MULTIPLEX-TRANSKRIPTOMANALYSE
Title (fr)
ANALYSE MULTIPLEX DE TRANSCRIPTOME
Publication
Application
Priority
- US 201462037575 P 20140814
- US 201462046845 P 20140905
- US 2015045209 W 20150814
Abstract (en)
[origin: WO2016025796A1] In some embodiments, the disclosure relates generally to methods, compositions, systems, apparatuses and kits comprising a multiplex nucleic acid amplification reaction that employs a plurality (e.g., hundreds, thousands, tens-of-thousands or hundreds-of-thousands) of different target-specific primer pairs that enable substantially simultaneous amplification of a plurality of different target sequences-of-interest in a single reaction mixture. In some embodiments, the multiplex nucleic acid amplification reaction generates a plurality of amplicons having sequences derived from a sample containing RNA or DNA, including whole transcriptome or genomic samples. In some embodiments, the sequences and abundances of at least some of the plurality of amplicons are characterized, optionally simultaneously or through a single assay, by suitable detection methods, including sequencing or other procedures known in the art.
IPC 8 full level
C12Q 1/68 (2006.01)
CPC (source: CN EP)
C12Q 1/6846 (2013.01 - CN EP); C12Q 1/6855 (2013.01 - CN EP)
C-Set (source: EP)
Citation (search report)
See references of WO 2016025796A1
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
Designated extension state (EPC)
BA ME
DOCDB simple family (publication)
WO 2016025796 A1 20160218; CN 107002123 A 20170801; EP 3180445 A1 20170621
DOCDB simple family (application)
US 2015045209 W 20150814; CN 201580054458 A 20150814; EP 15753859 A 20150814