Global Patent Index - EP 3196308 A1

EP 3196308 A1 20170726 - REPORTER VECTOR FOR EVALUATING CHARACTERISTICS OF SUBJECT CELL, ASSAY KIT, PROCEDURE AND DEVICE

Title (en)

REPORTER VECTOR FOR EVALUATING CHARACTERISTICS OF SUBJECT CELL, ASSAY KIT, PROCEDURE AND DEVICE

Title (de)

REPORTERVEKTOR ZUR BEURTEILUNG DER EIGENSCHAFTEN EINER SUBJEKTZELLE, TEST-KIT, VERFAHREN UND VORRICHTUNG

Title (fr)

VECTEUR-RAPPORTEUR DESTINÉ À ÉVALUER LES CARACTÉRISTIQUES D'UNE CELLULE DE SUJET, KIT DE DOSAGE, PROCÉDÉ ET DISPOSITIF

Publication

EP 3196308 A1 20170726 (EN)

Application

EP 17152059 A 20170118

Priority

JP 2016008125 A 20160119

Abstract (en)

According to one embodiment, a reporter vector includes a first and a second reporter gene expression unit (13, 25) and a replication initiation sequence (30). The first reporter gene expression unit (13) includes a promoter sequence of a cell characteristics marker (10), a first reporter gene (11), and a first transcription termination sequence (12). The second reporter gene expression unit (25) includes a promoter sequence exhibiting constitutive activity (20), a second reporter gene (21), a bicistronic expression sequence (22), a replication initiation protein gene (23), and a second transcription termination sequence (24). The replication initiation sequence (30) binds to a replication initiation protein, thereby initiating replication of the reporter vector.

IPC 8 full level

C12N 15/63 (2006.01); C12N 15/79 (2006.01); G01N 33/00 (2006.01)

CPC (source: EP US)

B01L 7/52 (2013.01 - US); C12N 15/63 (2013.01 - EP US); C12N 15/79 (2013.01 - EP US); C12N 15/86 (2013.01 - US); C12Q 1/6825 (2013.01 - US); C12Q 1/6886 (2013.01 - US); B01L 2300/0627 (2013.01 - US); B01L 2300/18 (2013.01 - US); C12N 2710/16143 (2013.01 - US); C12N 2710/22043 (2013.01 - US); C12N 2770/32243 (2013.01 - US); C12Q 2600/158 (2013.01 - US)

Citation (applicant)

JP 2012200245 A 20121022 - TOSHIBA CORP

Citation (search report)

  • [Y] WO 2014185549 A1 20141120 - TOSHIBA KK [JP], et al & EP 2998408 A1 20160323 - TOSHIBA KK [JP], et al
  • [IP] JP 2016101161 A 20160602 - TOSHIBA MEDICAL SYS CORP
  • [A] WO 2013157020 A1 20131024 - INDIAN INST TECHNOLOGY KANPUR [IN]
  • [Y] MATTHEW MAHON: "Vectors bicistronically linking a gene of interest to the SV40 large T antigen in combination with the SV40 origin of replication enhance transient protein expression and luciferase reporter activity", BIOTECHNIQUES RAPID DISPATCHES, 1 August 2011 (2011-08-01), US, XP055295383, ISSN: 0736-6205, DOI: 10.2144/000113720
  • [AP] ANONYMOUS: "pmirGLO Dual-Luciferase miRNA Target Expression Vector", 1 August 2016 (2016-08-01), XP002770678, Retrieved from the Internet <URL:https://www.promega.com/-/media/files/resources/protocols/product-information-sheets/a/pmirglo-dual-luciferase-mirna-target-expression-vector-protocol.pdf> [retrieved on 20170531]
  • [A] HE JIEYU ET AL: "Nampt/Visfatin/PBEF: A functionally multi-faceted protein with a pivotal role in malignant tumors", CURRENT PHARMACEUTICAL DESIGN, vol. 18, no. 37, 1 December 2012 (2012-12-01), NL, pages 6123 - 6132, XP055171101, ISSN: 1381-6128, DOI: 10.2174/138161212803582531

Designated contracting state (EPC)

AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

Designated extension state (EPC)

BA ME

DOCDB simple family (publication)

EP 3196308 A1 20170726; EP 3196308 B1 20190814; JP 2017127227 A 20170727; JP 6723011 B2 20200715; US 2017204469 A1 20170720

DOCDB simple family (application)

EP 17152059 A 20170118; JP 2016008125 A 20160119; US 201715410401 A 20170119