EP 3212778 A4 20180502 - METHODS AND COMPOSITIONS FOR STABILIZING TRANS-SPLICING INTEIN MODIFIED PROTEASES
Title (en)
METHODS AND COMPOSITIONS FOR STABILIZING TRANS-SPLICING INTEIN MODIFIED PROTEASES
Title (de)
VERFAHREN UND ZUSAMMENSETZUNGEN ZUR STABILISIERUNG VON TRANSSPLEISSENDEN INTEINMODIFIZIERTEN PROTEASEN
Title (fr)
PROCÉDÉS ET COMPOSITIONS DE STABILISATION DE PROTÉASES DE TRANS-ÉPISSAGE MODIFIÉE PAR INTÉINE
Publication
Application
Priority
- US 201462069653 P 20141028
- US 2015057862 W 20151028
Abstract (en)
[origin: WO2016069774A1] Methods for producing a trans-splicing intein-modified protease with enhanced solubility and regulating its activity are described. Intein-modified proteases having enhanced solubility and polynucleotides encoding the same are provided. Methods of storing trans-splicing proteases are also described.
IPC 8 full level
C12N 9/50 (2006.01); C11D 3/386 (2006.01); C12N 9/54 (2006.01); C12N 15/62 (2006.01)
CPC (source: EP US)
C07K 14/00 (2013.01 - US); C11D 3/386 (2013.01 - EP US); C11D 3/38663 (2013.01 - US); C12N 9/54 (2013.01 - EP US); C07K 2319/00 (2013.01 - EP US); C07K 2319/92 (2013.01 - EP US)
Citation (search report)
- [Y] WO 2014055782 A1 20140410 - AGRIVIDA INC [US]
- [Y] US 2014106399 A1 20140417 - LIN ZHANGLIN [CN], et al
- [Y] YONG-GANG XIE ET AL: "Effects of Thioredoxin: SUMO and Intein on Soluble Fusion Expression of an Antimicrobial Peptide OG2 in Escherichia coli", PROTEIN & PEPTIDE LETTERS, 1 January 2013 (2013-01-01), Netherlands, pages 54 - 60, XP055463003, Retrieved from the Internet <URL:http://www.eurekaselect.com/104989/article> DOI: 10.2174/092986613804096775
- [Y] ZHONGYUAN WANG ET AL: "Ubiquitin-intein and SUMO2-intein fusion systems for enhanced protein production and purification", PROTEIN EXPRESSION AND PURIFICATION, VOL. 82, N.1, 8 December 2011 (2011-12-08), pages 174 - 178, XP055436032, Retrieved from the Internet <URL:http://api.elsevier.com/content/article/PII:S1046592811003445?httpAccept=text/plain> [retrieved on 20171218], DOI: 10.1016/j.pep.2011.11.017
- See also references of WO 2016069774A1
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
DOCDB simple family (publication)
WO 2016069774 A1 20160506; BR 112017008938 A2 20180116; BR 112017008938 B1 20230926; CA 2963148 A1 20160506; CA 2963148 C 20240430; CN 107075491 A 20170818; CN 107075491 B 20210706; DK 3212778 T3 20191104; EP 3212778 A1 20170906; EP 3212778 A4 20180502; EP 3212778 B1 20190828; US 10731143 B2 20200804; US 11066657 B2 20210720; US 2018298368 A1 20181018; US 2020308564 A1 20201001
DOCDB simple family (application)
US 2015057862 W 20151028; BR 112017008938 A 20151028; CA 2963148 A 20151028; CN 201580058516 A 20151028; DK 15854157 T 20151028; EP 15854157 A 20151028; US 201515519602 A 20151028; US 202016899064 A 20200611